Human IL-4 ELISpot Set
Product Specifications
- Catalogue N°
- 856.011.001 - 1 x 96 Discovery (plate not included)
856.011.001P - 1 x 96 Discovery (non-sterile plate)
856.011.001S - 1 x 96 Discovery (sterile plate)
856.011.005 - 5 x 96 (plates not included)
856.011.005P - 5 x 96 (non-sterile plates)
856.011.005S - 5 x 96 (sterile plates)
856.011.010 - 10 x 96 (plates not included)
856.011.010P - 10 x 96 (non-sterile plates)
856.011.010S - 10 x 96 (sterile plates)
856.011.015 - 15 x 96 (plates not included)
856.011.015P - 15 x 96 (non-sterile plates)
856.011.015S - 15 x 96 (sterile plates)
856.011.020 - 20 x 96 (plates not included)
856.011.020P - 20 x 96 (non-sterile plates)
856.011.020S - 20 x 96 (sterile plates) - Target species
- Human
- Specificity
- Recognizes natural human IL-4
- Incubation
- 3h after cell stimulation
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB and blocking reagent.
- Additional Information
- Spots read visually or with reader
References
- Gupta, R. et al., Reprod Biol Endocrinol.,2009; 7: 38 - Pubmed link
- Hudak S. et al., J. Immuno., 2002; 169(3): 1189 - 1196 - Pubmed link
- Kalogerakou, F. et al.,Hippokratia,2008; 12(4): 230-5. - Pubmed link
- Sauce D. et al., Blood, 2002; 99(4) : 1165 - 1173 - Pubmed link
Related products
- 855.030.000 - Anti-Human IL-4 Azide Free
- 855.172.010 - Anti-Human IL-4 PE Conjugated
- 879.020.001 - Anti-Human IL-4 Capture Antibody
- 879.020.002 - Anti-Human IL-4 Detection Antibody
- 660.020.096 - Murine IL-4 ELISA Kit
- 850.890.048 - Human IL-4 High Sensitivity ELISA Kit
- 865.020.048 - Rat IL-4 ELISA Kit
- 950.020.048 - Human IL-4 ELISA Kit
- 851.510.001 - Human IL-4 ELISA Set
- 872.020.001 - Rat IL-4 ELISA Set
- 856.011.001PC - Human IL-4 ELISpot Kit
- 869.010.010 - Human IL-4 ELISpot Pair
- 855.170.000 - Anti-Human IL-4 Azide Free
- 853.800.000 - Anti-Human IL-4 Azide Free
BACKGROUND
IL-4 is a lymphokine that co-stimulates the proliferation of activated B- and T-cells, augments the cytotoxic activity of lymphocytes and monocytes and enhances the functional activity of myeloid cells. Produced by mast cells, T-cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ cells into helper Th2 cells characterized by their cytokine secretion-profile that includes secretion of IL-4, IL-5, IL-6, IL-10 and IL-13 which favour a humoral immune response. In addition, IL-4 can inhibit the proliferation of TNF, IL-1 and IL-6 by macrophages.
IL-4 induces B-cell class switching to IgE and IgG1 isotypes, and up regulates MHC Class II production and CD23 expression.
IL-4 is a 15kDa globular glycoprotein containing 129 amino acid residues. The non-glycosylated form of the protein is fully biologically active.
Version 9 - 07.19
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.