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Human IL-4 ELISA Kit

CE certified product *

Product Specifications

Catalogue N°
950.020.048 - 1 x 48 Discovery (pre-coated)
950.020.096 - 1 x 96 (pre-coated)
950.020.192 - 2 x 96 (pre-coated)
Assay Range
1.1 pg/ml - 35 pg/ml
Sensitivity
0.5 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human IL-4
Incubation
3h50
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
IL-4 ELISA Kit
IL-4 ELISA Kit

BACKGROUND

IL-4 mediates ist function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M.

Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4.

IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFNg, and lymphotoxin (TNFb). Apart from T cells, it has been shown that mast cells can produce IL-4.

IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells.

In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

Version 21 - 07.19

For research use only

Documents

* CE marked products are available for IVD use in the EU only. For all other regions these products are for research use only.

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

950.020.048 1 x 48 Discovery (pre-coated)
950.020.096 1 x 96 (pre-coated)
950.020.192 2 x 96 (pre-coated)

Ordering options

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