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Rat IL-4 ELISA Set

Product Specifications

Catalogue N°
872.020.001 - 1 x 96 Discovery
872.020.005 - 5 x 96
872.020.010 - 10 x 96
872.020.015 - 15 x 96
872.020.020 - 20 x 96
Assay Range
31.25 pg/ml - 1000 pg/ml
Sensitivity
15 pg/ml
Target species
Rat
Specificity
Recognizes both natural and recombinant rat IL-4
Incubation
From sample to end 4h45
Sample Type
Serum, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other rat cytokines
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

BACKGROUND

IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M.

Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4.

IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFNg, and lymphotoxin (TNFb). Apart from T cells, it has been shown that mast cells can produce IL-4.

IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells.

In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

Version 7 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

872.020.001 1 x 96 Discovery
872.020.005 5 x 96
872.020.010 10 x 96
872.020.015 15 x 96
872.020.020 20 x 96

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