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Murine IL-4 ELISA Kit

Product Specifications

Catalogue N°
660.020.096 - 1 x 96 (pre-coated)
660.020.192 - 2 x 96 (pre-coated)
Assay Range
3.9 pg/ml - 250 pg/ml
Sensitivity
3 pg/ml
Target species
Murine
Specificity
Recognizes both natural and recombinant murine IL-4
Incubation
3h20
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
50 µl
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
mIL-4 ELISA Kit
mIL-4 ELISA Kit

BACKGROUND

IL-4 mediates ist function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M.

Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4.

IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFNg, and lymphotoxin (TNFb). Apart from T cells, it has been shown that mast cells can produce IL-4.

IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells.

In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

Version 3 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

660.020.096 1 x 96 (pre-coated)
660.020.192 2 x 96 (pre-coated)

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