Human Perforin EasySplit ELISpot Kit

Product Specifications

Catalogue N°

856.141.001PCS - 1 x 96 Discovery (pre-coated strip plate)
856.141.002PCS - 2 x 96 (pre-coated strip plates)
856.141.005PCS - 5 x 96 (pre-coated strip plates)

Target species

Human

Specificity

Recognizes natural human Perforin

Incubation

3h after cell stimulation

Kit Content

Diaclone EasySplit ELISpot kits include: 
Pre-coated strip plate
Detection biotinylated antibody
Streptavidin Alkaline Phosphatase Conjugate
Bovine Serum Albumin
Ready-to-use BCIP/NBT Substrate Buffer

• References
• Related products

BACKGROUND

Perforin is a 70kDa protein containing 534 amino acid.

Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.

Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Its role in the immune response against tumors and virus infection has been demonstrated.

Version 1 - 07.23

For research use only

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