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Human Perforin ELISA Set

Product Specifications

Catalogue N°
851.860.001 - 1 x 96 Discovery
851.860.005 - 5 x 96
851.860.010 - 10 x 96
851.860.015 - 15 x 96
851.860.020 - 20 x 96
Assay Range
62.5 pg/ml - 2000 pg/ml
Sensitivity
40 pg/ml
Target species
Human
Specificity
Recognizes natural human Perforin
Incubation
From sample to end 2h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human soluble molecules
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

BACKGROUND

Perforin is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and natural Killer cells (NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell.

Perforin is one of the major effector molecules used by cytotoxic T cells and Natural Killers cells to mediate targeted cell lysis.

Version 8 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

851.860.001 1 x 96 Discovery
851.860.005 5 x 96
851.860.010 10 x 96
851.860.015 15 x 96
851.860.020 20 x 96

Ordering options

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