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Human Perforin ELISA Set

Product Specifications

Catalogue N°
851.860.001 - 1 x 96 Discovery
851.860.005 - 5 x 96
851.860.010 - 10 x 96
851.860.015 - 15 x 96
851.860.020 - 20 x 96
Assay Range
62.5 pg/ml - 2000 pg/ml
Sensitivity
40 pg/ml
Target species
Human
Specificity
Recognizes natural human Perforin
Incubation
From sample to end 2h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human soluble molecules
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

BACKGROUND

Perforin is a 70kDa protein containing 534 amino acid.

Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.

Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Its role in the immune response against tumors and virus infection has been demonstrated.

Version 9 - 04.20

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

851.860.001 1 x 96 Discovery
851.860.005 5 x 96
851.860.010 10 x 96
851.860.015 15 x 96
851.860.020 20 x 96

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