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Human Perforin ELISA Kit

Product Specifications

Catalogue N°
850.860.048 - 1 x 48 Discovery (pre-coated)
850.860.096 - 1 x 96 (pre-coated)
850.860.192 - 2 x 96 (pre-coated)
Assay Range
62.5 pg/ml - 2000 pg/ml
Sensitivity
40 pg/ml
Target species
Human
Specificity
Recognizes natural human Perforin
Incubation
2h45
Sample Type
Supernatant, Buffered Solutions, Not suitable for use with serum and plasma.
Sample Size
100 µl
Cross Reaction
No cross reactivity with Trail, TRAIL R3, TRAIL R4, Granzyme B, TNFα, IFNγ, Fas, Fas L, IL-1b, IL-2
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Perforin ELISA Kit
Perforin ELISA Kit

BACKGROUND

Perforin is a pore forming cytolytic protein found in the granules of cytotoxic T lymphocytes (CTLs) and natural Killer cells (NK cells. Upon degranulation, perforin binds to the target cell's plasma membrane, and oligomerises in a Ca2+ dependent manner to form pores on the target cell. The pore formed allows for the passive diffusion of a family of pro-apoptotic proteases, known as the granzymes, into the target cell.

Perforin is one of the major effector molecules used by cytotoxic T cells and Natural Killers cells to mediate targeted cell lysis.

Version 12 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

850.860.048 1 x 48 Discovery (pre-coated)
850.860.096 1 x 96 (pre-coated)
850.860.192 2 x 96 (pre-coated)

Ordering options

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