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Anti-Human Perforin FITC Conjugated

Product Specifications

Catalogue N°
854.951.010 - 100 tests / 1ml
Target species
Human
Specificity
Recognizes human Perforin, a 70 kDa protein
Clone
B-D48
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
Natural human Perforin
Quantity
100 Tests / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline with 5% BSA and 0.09% sodium azide
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
Use 10 μl to label 106 cells or 100 μl of whole blood
Anti-Human Perforin FITC
A typical staining pattern with the B-D48 monoclonal antibody of lymphocytes

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.

Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Perforin is a 70kDa protein containing 534 amino acid.

Its role in the immune response against tumors and virus infection has been demonstrated.

Version 11 - 07.19

For research use only

Documents

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Formats Available

854.951.010 100 tests / 1ml

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