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Human Perforin ELISpot Set

Product Specifications

Catalogue N°
856.141.001 - 1 x 96 Discovery (plate not included)
856.141.001P - 1 x 96 Discovery (non-sterile plate)
856.141.001S - 1 x 96 Discovery (sterile plate)
856.141.005 - 5 x 96 (plates not included)
856.141.005P - 5 x 96 (non-sterile plates)
856.141.005S - 5 x 96 (sterile plates)
856.141.010 - 10 x 96 (plates not included)
856.141.010P - 10 x 96 (non-sterile plates)
856.141.010S - 10 x 96 (sterile plates)
856.141.015 - 15 x 96 (plates not included)
856.141.015P - 15 x 96 (non-sterile plates)
856.141.015S - 15 x 96 (sterile plates)
856.141.020 - 20 x 96 (plates not included)
856.141.020P - 20 x 96 (non-sterile plates)
856.141.020S - 20 x 96 (sterile plates)
Target species
Human
Specificity
Recognizes natural human Perforin
Incubation
3h after cell stimulation
Kit Content
Diaclone ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB and blocking reagent.
Additional Information
Spots read visually or with reader

BACKGROUND

Perforin is involved in the killing function by CTLs and NKs. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. It leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program.

Perforin has structural and functional similarities to complement component 6, 7, 8 and 9 (C6, C7, C8, C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.

Perforin is a 70kDa protein containing 534 amino acid.

Its role in the immune response against tumors and virus infection has been demonstrated.

Version 7 - 07.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

856.141.001 1 x 96 Discovery (plate not included)
856.141.001P 1 x 96 Discovery (non-sterile plate)
856.141.001S 1 x 96 Discovery (sterile plate)
856.141.005 5 x 96 (plates not included)
856.141.005P 5 x 96 (non-sterile plates)
856.141.005S 5 x 96 (sterile plates)
856.141.010 10 x 96 (plates not included)
856.141.010P 10 x 96 (non-sterile plates)
856.141.010S 10 x 96 (sterile plates)
856.141.015 15 x 96 (plates not included)
856.141.015P 15 x 96 (non-sterile plates)
856.141.015S 15 x 96 (sterile plates)
856.141.020 20 x 96 (plates not included)
856.141.020P 20 x 96 (non-sterile plates)
856.141.020S 20 x 96 (sterile plates)

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