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Human IL-10 ELISpot Pair

Product Specifications

Catalogue N°
869.030.010 - 10 x 96 tests
Target species
Human
Specificity
Recognizes natural human IL-10
Cross Reaction
No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
Kit Content
Diaclone ELISpot matched antibody pairs are extensively validated and include pre-titrated capture antibody and biotinylated detection antibody. Antibodies are supplied in quantities sufficient for 10 x 96 samples.

BACKGROUND

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells.In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exists at 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome.

The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

IL-10 in disease

IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni , Leishmania, Toxoplasma gondii and Trypanosoma infection.

Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae, Mycobacterium tuberculosis and Mycobacterium avium infections.

High expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency.

Version 4 - 07.19

For research use only

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Formats Available

869.030.010 10 x 96 tests

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