Anti-Human IL-10 Azide Free
Product Specifications
- Catalogue N°
- 855.100.000 - 200µg / 200µl
855.100.005 - 500µg / 500µl - Target species
- Human
- Specificity
- Recognises both natural and recombinant human IL-10
- Clone
- B-S10
- Application
- ELISA
- Immunohistochemistry
- Functional assay
- Hybridoma
- Myeloma X63/AG.8653 x Balb/c spleen cells
- Immunisation
- Recombinant human IL-10
- Quantity
- 200µg or 500µg (Discovery Size also available please enquire)
- Isotype
- Mouse IgG1 Kappa light chain
- Format
- Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free
- Storage
- Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
- Biological Activity
- Blocks IL-10 induced inhibition of IL-6 synthesis on LPS activated monocytes
References
- Balashov, K. E. et al., Neurology,2000; 55(2): 192-8. - Pubmed link
- Brok, H. P. et al., J Immunol.,2002; 169(11): 6554-63. - Pubmed link
- Debili, N.et al. Blood,2001; 97(7): 2023-2030. - Pubmed link
- Egeler, R. M. et al., Blood,1999; 94(12): 4195-201. - Pubmed link
- Fontana, S. et al., Blood,2003; 102(12): 4099-106. - Pubmed link
- Jarry, A. et al., J. Clin. Invest., 2008;118(3): 1132-42 - Pubmed link
- Kap, Y. S. et al., Journal of Histochemistry & Cytochemistry,2009;57(12):1159-1167. - Pubmed link
- Knol, A. C. et al.,PLoS One,2012;7(12): e48729 - Pubmed link
- Lelievre, E. et al., Cytokine,1998; 10(11): 831-40. - Pubmed link
- Naji, A. et al., Blood,2007;110(12):3936-48 - Pubmed link
- Ravirajan C.T. et al., 2004; 43(4) : 442 - 447 - Pubmed link
- Schandene, L. et al., J Immunol.,1994; 152(9): 4368-74. - Pubmed link
- Schrijver, I. A. et al., Brain,2001; 124(Pt 8): 1544-54. - Pubmed link
- Shinagawa, S. et al.,Sci Rep., 2017 Nov 3;7(1):14500 - Pubmed link
- Villoslada, P. et al., J Exp Med.,2000; 191(10): 1799-806. - Pubmed link
- Proust R et al., Stem Cell Res Ther. 2020; 11: 172. - Pubmed link
- Mestrallet, G. et al., Front Immunol. 2022; 13: 786859. - Pubmed link
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- 850.880.048 - Human IL-10 High Sensitivity ELISA Kit
- 860.030.048 - Murine IL-10 ELISA Kit
- 950.060.048 - Human IL-10 ELISA Kit
- 851.540.001 - Human IL-10 ELISA Set
- 861.030.001 - Murine IL-10 ELISA Set
- 869.030.010 - Human IL-10 ELISpot Pair
- 670.070.096 - Rat IL-10 ELISA kit
- 853.870.000 - Anti-Human IL-10 Azide Free
- 879.100.001 - Anti-Human IL-10 Capture Antibody
BACKGROUND
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine.
The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates . Recombinant protein expressed in E. coli thus retains all known biological activities.The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host- virus interaction.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
IL-10 in disease
IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni, Leishmania, Toxoplasma gondii and Trypanosoma infection. Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae, Mycobacterium tuberculosis and Mycobacterium avium infections. High expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency.
Version 16 - 09.22
For research use only
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