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Human IL-10 ELISA Kit

CE certified product *

Product Specifications

Catalogue N°
950.060.048 - 1 x 48 Discovery (pre-coated)
950.060.096 - 1 x 96 (pre-coated)
950.060.192 - 2 x 96 (pre-coated)
Assay Range
12.5 pg/ml - 400 pg/ml
Sensitivity
5 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human IL-10
Incubation
2h40
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
IL-10 ELISA Kit
IL-10 ELISA Kit

BACKGROUND

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells.

In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa.

Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates . Recombinant protein expressed in E. coli thus retains all known biological activities.The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host- virus interaction.

The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

IL-10 in disease

IL-10 expression was shown to be elevated in parasite infections like in Schistosoma mansoni, Leishmania, Toxoplasma gondii and Trypanosoma infection. Furthermore, high IL-10 expression was detected in mycobacterial infections as shown for Mycobacterium leprae, Mycobacterium tuberculosis  and Mycobacterium avium infections. High expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency.

Version 31 - 02.20

For research use only

Documents

* CE marked products are available for IVD use in the EU only. For all other regions these products are for research use only.

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

950.060.048 1 x 48 Discovery (pre-coated)
950.060.096 1 x 96 (pre-coated)
950.060.192 2 x 96 (pre-coated)

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