- Catalogue N°
- 860.030.048 - 1 x 48 Discovery (pre-coated)
860.030.096 - 1 x 96 (pre-coated)
860.030.192 - 2 x 96 (pre-coated)
- Assay Range
- 31.25 pg/ml - 1000 pg/ml
- 20 pg/ml
- Target species
- Recognizes both natural and recombinant murine IL-10
- Sample Type
- Serum, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other murine cytokines
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. Based on its primary structure, IL-10 is a member of the four -helix bundle family of cytokines. In solution IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked glycosylation site, it lacks detectable carbohydrates.
Recombinant protein expressed in E. coli thus retains all known biological activities. The IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The murine IL-10 exhibits strong DNA and amino acid sequence homology to the human IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.
Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Version 10 - 07.19
For research use only
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