Search for products

Anti-Human IFN-γ Azide Free

Product Specifications

Catalogue N°
855.000.000 - 200µg / 200µl
855.000.005 - 500µg / 500µl
Target species
Recognises both natural and recombinant human IFN-g
Flow Cytometry
Functional assay
Myeloma X63/AG.8653 x Balb/c spleen cells
Recombinant human IFN-g
200µg or 500µg (Discovery Size also available please enquire)
Mouse IgG1 Kappa light chain
Phosphate-buffered saline. Sterile-filtered through 0.22 µm. Carrier and preservative free
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Biological Activity
Inhibits IFNγ induced proliferation
Anti-Human IFN-γ Azide Free
The graph on the left represents activated lymphocytes marked with B-B1.
Anti-Human IFN-γ Azide Free
The graph on the right shows B-B1 with non-activated lymphocytes. In the two cases, the dotted line represents the IgG1 isotype control.


Interferon-gamma (IFN-γ, IFN-g, IFN gamma), also known as type II or immune interferon, is a dimerized soluble cytokine.

Interferon gamma is produced mainly by activated T cells and Natural Killer cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops. IFN gamma is also produced by non-cytotoxic innate lymphoid cells (ILC), a family of immune cells first discovered in the early 2010s

IFNg is considered to be a proinflammatory cytokine, that activates macrophages and endothelial cells, it also regulates immune responses by effecting APC, and T cells.

IFN gamma is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFN gamma is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFNγ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects.

Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFN is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.

IFN is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNγ during an immune response will result in the preferential proliferation of Th1 cells.

In addition, IFNγ has several properties related to immunoregulation. IFNγ is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFα. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.

The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.

Version 11 - 04.20

For research use only


For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

855.000.000 200µg / 200µl
855.000.005 500µg / 500µl

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email:

Please contact us to discuss bulk orders