872.000.001 - 1 x 96 Discovery 872.000.005 - 5 x 96 872.000.010 - 10 x 96 872.000.015 - 15 x 96 872.000.020 - 20 x 96
Assay Range
31.25 pg/ml - 1000 pg/ml
Sensitivity
10 pg/ml
Target species
Rat
Specificity
Recognizes both natural and recombinant rat IFN-g
Incubation
From sample to end 3h35
Sample Type
Serum, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other rat cytokines
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFNg is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.
IFNg is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNγ during an immune response will result in the preferential proliferation of Th1 cells.
In addition, IFNγ has several properties related to immunoregulation. IFNγ is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFα. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.
The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetesand allergy.
