Rat IFN-γ ELISA Set
Product Specifications
- Catalogue N°
- 872.000.001 - 1 x 96 Discovery
872.000.005 - 5 x 96
872.000.010 - 10 x 96
872.000.015 - 15 x 96
872.000.020 - 20 x 96 - Assay Range
- 31.25 pg/ml - 1000 pg/ml
- Sensitivity
- 10 pg/ml
- Target species
- Rat
- Specificity
- Recognizes both natural and recombinant rat IFN-g
- Incubation
- From sample to end 3h35
- Sample Type
- Serum, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other rat cytokines
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
- Synonym
- IFN-g
- IFN-gamma
References
- Mirza, S. et al., Cell Stress Chaperones,2006; 11(4): 364-78. - Pubmed link
- Schif-Zuck S. et al., J. Immunol., 2005, 174(7): 4307 - 4315 - Pubmed link
- Slack, L. K. et al.,Cell Stress Chaperones,2007; 12(1): 71-82. - Pubmed link
Related products
- 855.000.000 - Anti-Human IFN-γ Azide Free
- 855.001.019 - Anti-Human IFN-γ FITC Conjugated
- 855.002.019 - Anti-Human IFN-γ PE Conjugated
- 879.000.001 - Anti-Human IFN-γ Capture Antibody
- 879.000.002 - Anti-Human IFN-γ Detection Antibody
- 850.900.048 - Human IFN-γ High Sensitivity ELISA Kit
- 860.050.048 - Murine IFN-γ ELISA Kit
- 865.010.048 - Rat IFN-γ ELISA Kit
- 950.000.048 - Human IFN-γ ELISA Kit
- 851.560.001 - Human IFN-γ ELISA Set
- 861.050.001 - Murine IFN-γ ELISA Set
- 856.051.001 - Human IFN-γ ELISpot Set
- 856.051.001PC - Human IFN-γ ELISpot Kit
- 862.031.001 - Murine IFN-γ ELISpot Set
- 862.031.001PC - Murine IFN-γ ELISpot Kit
- 867.011.001 - Rat IFN-γ ELISpot Set
- 867.011.001PC - Rat IFN-γ ELISpot Kit
- 869.060.010 - Human IFN-γ ELISpot Pair
- 870.050.010 - Murine IFN-γ ELISpot Pair
- 871.020.010 - Rat IFN-γ ELISpot Pair
- 853.670.000 - Anti-Human IFN-γ Azide Free
- 858.000.005 - Accessory Pack for ELISA set
BACKGROUND
Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFNg is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.
IFNg is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNγ during an immune response will result in the preferential proliferation of Th1 cells.
In addition, IFNγ has several properties related to immunoregulation. IFNγ is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFα. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.
The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.
Version 7 - 07.19
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.