Rat IFN-γ ELISA Set

Product Specifications

Catalogue N°

872.000.001 - 1 x 96 Discovery
872.000.005 - 5 x 96
872.000.010 - 10 x 96
872.000.015 - 15 x 96
872.000.020 - 20 x 96

Assay Range

31.25 pg/ml - 1000 pg/ml

Sensitivity

10 pg/ml

Target species

Rat

Specificity

Recognizes both natural and recombinant rat IFN-g

Incubation

From sample to end 3h35

Sample Type

Serum, Cell culture supernatant

Sample Size

100 µl

Cross Reaction

No cross reactivity with other rat cytokines

Kit Content

Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

Synonym

IFN-g

IFN-gamma

• References
• Related products

BACKGROUND

Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFNg is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.

IFNg is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNγ during an immune response will result in the preferential proliferation of Th1 cells.

In addition, IFNγ has several properties related to immunoregulation. IFNγ is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFα. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.

The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.

Version 7 - 07.19

For research use only

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