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Rat IFN-γ ELISpot Kit

Product Specifications

Catalogue N°
867.011.001PC - 1 x 96 Discovery (pre-coated plate)
867.011.005PC - 5 x 96 (pre-coated plates)
Target species
Rat
Specificity
Recognizes natural rat IFN-g
Incubation
3h after cell stimulation
Cross Reaction
No cross reactivity with other rat cytokines
Kit Content
Diaclone Pre-coated ELISpot kits include precoated PVDF plates, Detection antibody, Alkaline phosphatase conjugate, BSA, BCIP/NBT ready-to-use substrate buffer.
Synonym
IFN-g
IFN-gamma

BACKGROUND

Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNg production is a key function of Th1, CD8+ CTLs and also NK cells. IFNg is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFNg is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.

IFNg is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFa and IFNg, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNg. IFNg preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNg during an immune response will result in the preferential proliferation of Th1 cells.

In addition, IFNg has several properties related to immunoregulation. IFNg is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFa. IFNg induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNg promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.

The role of IFNg as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, Autoimmune disease, transplant rejection, Diabetes and allergy.

Version 4 - 07.19

For research use only

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Formats Available

867.011.001PC 1 x 96 Discovery (pre-coated plate)
867.011.005PC 5 x 96 (pre-coated plates)

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