Recognises both natural and recombinant human IP-10
Clone
B-C50
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c node cells
Immunisation
Recombinant human IP-10
Quantity
100 Tests / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG2a Kappa light chain
Format
Phosphate-buffered saline with 5 % BSA and 0.1 % sodium azide
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
Use 10 μl to label 106 cells or 100 μl of whole blood
Synonym
CXCL10
We used U937 cells 3 days after passaging. Half were activated with IFN-gamma 100 ng/ml for 20 hours and half were used as control. Cell concentration at the beginning of activation was 2.105 cells/ml. Brefaldin A was used as an intracellular blocking agent at 1μg/ml, in contact with the cells for 4 to 6 hours. The black line on the graph represents the IgG2b isotype control (B-E4), the green line shows B-C50 with U937 non-activated cells and the yellow line represents activated U937 cells marked with B-C50 (10μl per test for 5.105 cells).
Sodium azide is a poisonous and hazardous substance, handle and dispose of properly
BACKGROUND
IP-10 (Interferon-gamma inducible Protein 10kDa) also known as CXCL10, is secreted by several cell types in response to IFNg and LPS. These cell types include monocytes, endothelial cells and fibroblasts.The gene for IP-10 is located on chromosome 4 in a cluster among several other cytokines and encodes a 98 amino acid precursor protein.
Several roles have been attributed to IP-10, such as chemoattraction for monocytes and T cells (but not for neutrophils), inhibition of bone marrow colony formation and angiogenesis, promotion of T cells adhesion molecule expression.
IP-10 shares a common receptor, CXCR3, with the chemokine MIG, but has been shown to play a distinct role in host defense in infections.
IP-10 expression has been associated with HIV infection, is involved in inflammatory skin disease and other allergic diseases; it appears in inflammation of the nervous system and in Alzheimer’s disease (astrocytes expressing IP-10 are commonly associated with senile plaques).
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Formats Available
853.571.019 100 tests / 1ml
Ordering options
Place an order by phone: +33 (0)3 81 41 38 38
or by email: info
[at]
diaclone [dot] com
(
Subject: Diaclone's order,
Body: Anti-Human IP-10 FITC Conjugated
853.571.019 - 100 tests / 1ml
)
Sodium azide is a poisonous and hazardous substance, handle and dispose of properly