- Catalogue N°
- 852.030.000 - 200µg / 200µl
852.030.005 - 500µg / 500µl
- Target species
- Recognises the Interleukin-6 Ra subunit, IL-6 Ra, IL-6 R, a 80 kDa protein
- Flow Cytometry
- Functional assay
- Immunohistochemistry on frozen sections
- Myeloma X63/AG.8653 x Balb/c spleen cells
- gp80 transfected CHO cell line
- 200µg or 500µg (Discovery Size also available please enquire)
- Mouse IgG1 Kappa light chain
- Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free
- Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
- Biological Activity
- Inhibits IL-6 induced proliferation of XG-1 cells
Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response.
The receptor complex mediating the biological activities consists of two distinct membrane-bound glycoproteins:
- IL-6 Receptor (IL-6R, gp80) is 80 kDa alpha subunit, transmembrane glycoprotein that is 449 aa long. It binds IL-6 with low affinity
- A signal-transducing non-ligand binding 130 kDa component (gp130) that is required for high-affinity binding complex (Kd = 50 pM)
Soluble IL-6 receptor (sIL-6R) is a 50-55 kDa ligand binding protein, derived from the extracellular part of the gp80 receptor by either proteolytic cleavage or by alternative splicing. The soluble form of IL-6R can bind its ligand and induce cellular responses by association with gp130, thus acting as an IL-6 agonist.
Dysregulated production of IL6 and its receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Elevated IL-6 concentrations have been reported in numerous clinical disorders where they appear to orchestrate a variety of inflammatory responses.
Version 11 - 05.21
For research use only
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