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Human CD126 ELISA kit

Product Specifications

Catalogue N°
850.510.048 - 1 x 48 Discovery (pre-coated)
850.510.096 - 1 x 96 (pre-coated)
850.510.192 - 2 x 96 (pre-coated)
Assay Range
31.25 pg/ml - 1000 pg/ml
Sensitivity
5 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human soluble IL-6R
Incubation
1h45
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human soluble receptors. No interference with neither soluble gp130 nor IL-6.
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
IL-6R
gp80
CD126 ELISA Kit
Example of Human CD126 Standard Curve

References

  • Arranz-Valsero, I. et al.,Mol Vis.,2013;19: 85-99 - Pubmed link
  • Burger, R. et al., Hematol J.,2001; 2(1): 42-53. - Pubmed link
  • Corbi P. et al., Eur J Cardiothorac Surg., 2000; 18(1): 98 - 103 - Pubmed link
  • Dore, P. et al., Clin Exp Immunol.,1997; 107(1): 182-8 - Pubmed link
  • Gombocz, K. et al., Crit Care,2007; 11(4): R87 - Pubmed link
  • Gougelet, A. et al., PLoS One,2009; 4(11): e8026 - Pubmed link
  • Klausz, K. et al.,Oncotarget, 2017 Sep 5;8(44):77552-77566. - Pubmed link
  • Le Meur Y. et al., Nephrol. Dial. Transplant., 1999; 14(10) : 2420 - 2426 - Pubmed link
  • Puthier, D. et al., Blood,1996; 88(12): 4659-66 - Pubmed link
  • Santer, F. R. et al., Endocr. Relat. Cancer, 2010;17(1): 241-253. - Pubmed link
  • Zekri, A. R. et al., World J Gastroenterol.,2005;11(42): 6624-30 - Pubmed link
  • Leifsdottir, KJ. et al., Neuroinflammation. 2018 Aug 8;15(1):223. - Pubmed link
  • Zhang, Z. Y. et al., Biomed Res Int. 2019; 2019: 1575468. - Pubmed link
  • Burger, R. et al., Haematologica. 2021 Sep 1; 106(9): 2507–2510. - Pubmed link

BACKGROUND

Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response.
The receptor complex mediating the biological activities consists of two distinct membrane-bound glycoproteins:

  • IL-6 Receptor (IL-6R, gp80) is 80 kDa alpha subunit, transmembrane glycoprotein that is 449 aa long. It binds IL-6 with low affinity
  • A signal-transducing non-ligand binding 130 kDa component (gp130) that is required for high-affinity binding complex (Kd = 50 pM)

Soluble IL-6 receptor (sIL-6R) is a 50-55 kDa ligand binding protein, derived from the extracellular part of the gp80 receptor by either proteolytic cleavage or by alternative splicing. The soluble form of IL-6R can bind its ligand and induce cellular responses by association with gp130, thus acting as an IL-6 agonist.

Dysregulated production of IL6 and its receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Elevated IL-6 concentrations have been reported in numerous clinical disorders where they appear to orchestrate a variety of inflammatory responses.

Version 15 - 02.22

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Formats Available

850.510.048 1 x 48 Discovery (pre-coated)
850.510.096 1 x 96 (pre-coated)
850.510.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human CD126 ELISA kit 850.510.048 - 1 x 48 Discovery (pre-coated) 850.510.096 - 1 x 96 (pre-coated) 850.510.192 - 2 x 96 (pre-coated) )

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