Anti-Human CD126 FITC Conjugated

Product Specifications

Catalogue N°

852.051.010 - 100 tests / 1ml

Target species

Human

Specificity

Recognises the Interleukin-6 Ra subunit, IL-6 Ra, IL-6 R, a 80 kDa protein

Clone

B-N12

Application

Flow Cytometry

Hybridoma

Myeloma X63/AG.8653 x Balb/c spleen cells

Immunisation

gp80 transfected CHO cell line

Quantity

100 Tests / 1ml (Discovery Size also available please enquire)

Isotype

Mouse IgG1 Kappa light chain

Format

Phosphate-buffered saline with 5 % BSA and 0.1 % sodium azide

Workshop

V-VI

Storage

Stable at +2-8°C for 12 months. For longer storage freeze aliquots.

Working Dilution

Use 10 μl to label 10⁶ cells or 100 μl of whole blood

Synonym

IL-6R

gp80

• References
• Related products

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly

BACKGROUND

Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response.
The receptor complex mediating the biological activities consists of two distinct membrane-bound glycoproteins:

• IL-6 Receptor (IL-6R, gp80) is 80 kDa alpha subunit, transmembrane glycoprotein that is 449 aa long. It binds IL-6 with low affinity
• A signal-transducing non-ligand binding 130 kDa component (gp130) that is required for high-affinity binding complex (Kd = 50 pM)

Soluble IL-6 receptor (sIL-6R) is a 50-55 kDa ligand binding protein, derived from the extracellular part of the gp80 receptor by either proteolytic cleavage or by alternative splicing. The soluble form of IL-6R can bind its ligand and induce cellular responses by association with gp130, thus acting as an IL-6 agonist.

Dysregulated production of IL6 and its receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Elevated IL-6 concentrations have been reported in numerous clinical disorders where they appear to orchestrate a variety of inflammatory responses.

Version 6 - 06.19

For research use only

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