Anti-Human CD130 Azide Free
Product Specifications
- Catalogue N°
- 852.060.000 - 200µg / 200µl
852.060.005 - 500µg / 500µl - Target species
- Human
- Specificity
- Recognises the Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors, a 130-140 kDa protein
- Clone
- B-R3
- Application
- ELISA
- Flow Cytometry
- Immunohistochemistry
- Functional assay
- Hybridoma
- Myeloma X63/AG.8653 x Balb/c spleen cells
- Immunisation
- Natural soluble gp130
- Quantity
- 200µg or 500µg (Discovery Size also available please enquire)
- Isotype
- Mouse IgG2a Kappa light chain
- Format
- Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free
- Workshop
- VI
- Storage
- Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
- Biological Activity
- Inhibits IL-6, IL-11, CNTF, LIF and OSM activities
- Synonym
- gp130
References
- Memoli, B., Nephrol. Dial. Transplant,2001; 16(6): A184-186.
- Aoki, Y. et al., Blood,2001; 98(10): 3042-9. - Pubmed link
- Auguste, P. et al., J Biol Chem.,1997; 272(25): 15760-4. - Pubmed link
- Blanchard F. et al., J. Biol. Chem., 1998; 273(33): 20886 -20893 - Pubmed link
- Burger R. et al., The Haematology Journal, 2001; 2(1): 42-53 - Pubmed link
- Burger, R. et al.,Haematologica, 2017;102(2) :381-390 - Pubmed link
- Chautard, E. et al.,Neuro Oncology,2010;12(5): 434-443 - Pubmed link
- Chevalier S. et al., J. Biol. Chem., 1996; 271(25): 14764-72 - Pubmed link
- Duplomb L. et al., Endocrinology, 2003; 144(12) : 5381 - 5389 - Pubmed link
- Gougelet, A. et al.,PLoS One,2009; 4(11): e8026 - Pubmed link
- Gu, Z. J. et al., Blood,1996; 88(10): 3972-86. - Pubmed link
- Gu, Z. J. et al., J Immunol Methods,1996; 190(1): 21-7. - Pubmed link
- Gu, Z. J. et al., Leukemia,2000; 14(1): 188-97. - Pubmed link
- Jover, R. et al., Faseb J.,2002; 16(13): 1799-801. - Pubmed link
- Kube D. et al., Blood, 2001; 98(3) : 762 - 770 - Pubmed link
- Kurth, I. et al. ,J Immunol.,2000; 164(1): 273-82. - Pubmed link
- Lebeau, B. et al., FEBS Lett.,1997; 407(2): 141-7 - Pubmed link
- Leslie, K. et al., Breast Cancer Res.,2010; 12(5): R80. - Pubmed link
- Magrangeas, F. et al. ,Biochem J.,2001; 353(Pt 2): 223-30. - Pubmed link
- Memoli, B. et al., J Am Soc Nephrol.,2005; 16(4): 1099-107. - Pubmed link
- Müller-Newen G. et al., J. Biol. Chem., 2000; 275 (7) : 4578 - 4586 - Pubmed link
- Olivier, C. et al., J Biol Chem.,2000; 275(8): 5648-56. - Pubmed link
- Taupin J-L. et al., J. Biol. Chem., 2001; 276(51) : 47975 - 47981 - Pubmed link
- Thiel, S. et al., Biochem.,1998; J 330 (Pt 1): 47-54. - Pubmed link
- Waetzig, G. H. et al.,J. Biol. Chem.,2010; 285(3):1781-1789 - Pubmed link
Related products
- 852.061.010 - Anti-Human CD130 FITC Conjugated
- 852.063.020 - Anti-Human CD130 Unconjugated
- 852.070.000 - Anti-Human CD130 Azide Free
- 852.080.000 - Anti-Human CD130 Azide Free
- 852.090.000 - Anti-Human CD130 Azide Free
- 852.100.000 - Anti-Human CD130 Azide Free
- 852.110.000 - Anti-Human CD130 Azide Free
- 879.520.001 - Anti-Human CD130 Capture Antibody
- 879.520.002 - Anti-Human CD130 Detection Antibody
- 850.520.048 - Human CD130 ELISA kit
- 853.860.000 - Anti-Human CD130 Azide Free
BACKGROUND
Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. It forms one subunit of the type I cytokine receptor within the IL-6 receptor family. The IL-6 receptor complex mediating the biological activities consists of two distinct membrane-bound glycoproteins:
- IL-6 Receptor (IL-6R, gp80) is 80 kDa alpha subunit, transmembrane glycoprotein that is 449 aa long. It binds IL-6 with low affinity
- gp130 the signal-transducing non-ligand binding 130 kDa component (gp130) that is required for high-affinity binding complex (Kd = 50 pM)
It is often referred to as the common gp130 subunit, and is important for signal transduction following cytokine engagement. As with other type I cytokine receptors, gp130 possesses a WSXWS amino acid motif that ensures correct protein folding and ligand binding. It interacts with Janus kinases to elicit an intracellular signal following receptor interaction with its ligand. Structurally, gp130 is composed of five fibronectin type-III domains and one immunoglobulin-like C2-type (immunoglobulin-like) domain in its extracellular portion.
Soluble IL-6 receptor (sIL-6R) is a 50-55 kDa ligand binding protein, derived from the extracellular part of the gp80 receptor by either proteolytic cleavage or by alternative splicing. The soluble form of IL-6R can bind its ligand and induce cellular responses by association with gp130, thus acting as an IL-6 agonist.
Dysregulated production of IL6 and its receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Elevated IL-6 concentrations have been reported in numerous clinical disorders where they appear to orchestrate a variety of inflammatory responses.
Version 14 - 05.21
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.