Anti-Human CD40 PE Conjugated
Product Specifications
- Catalogue N°
- 954.282.010 - 100 tests / 1ml
- Target species
- Human
- Specificity
- Recognises the Bp50 antigen, a 48 kDa protein
- Clone
- B-B20
- Application
- Flow Cytometry
- Hybridoma
- Myeloma X63/AG.8653 x Balb/c spleen cells
- Immunisation
- Raji cell line
- Quantity
- 100 Tests / 1ml (Discovery Size also available please enquire)
- Isotype
- Mouse IgG1 Kappa light chain
- Format
- Phosphate-buffered saline with 1% BSA and 0.1% sodium azide
- Workshop
- VI
- Storage
- Stable at +2-8°C for six months after reconstitution. DO NOT FREEZE.
- Working Dilution
- Reconstitute with 1 ml deionised water. Use 10μl to label 106 cells or 100 μl of whole blood.
References
- Airoldi, I. et al., Br J Cancer,2003; 88(10): 1527-36 - Pubmed link
- Airoldi, I. et al., Br J Cancer,2004; 90(11): 2210-8. - Pubmed link
- Brok, H. P. et al., Cytometry,2001; 45(4): 294-303 - Pubmed link
- Butler, M. et al.,Immunology,2007;120(3): 362-71. - Pubmed link
- Garnache-Ottou F. et al., Blood, 2005; 105(3):1256 - 1264 - Pubmed link
- Iwamoto, S. et al., J Leukoc Biol.,2005; 78(2): 383-92. - Pubmed link
- Iwamoto, S. et al., J. Immunol.,2007; 179(3): 1449-1457. - Pubmed link
- Jego, G. et al., Blood,1999; 94(2): 701-12. - Pubmed link
- Kohrgruber, N. et al., J Immunol.,1999; 163(6): 3250-9. - Pubmed link
- Metcalfe, R. A. et al., J Clin Endocrinol Metab.,1998; 83(4): 1268-74. - Pubmed link
- Morandi, F. et al., Neoplasia,2006;8(10):833-42 - Pubmed link
- Raffaghello, L. et al., Cancer Res.,2007; 67(11): 5471-5478. - Pubmed link
BACKGROUND
CD40 belongs to the TNF receptor superfamily. While the biological role of some of the ligand-receptor pairs in this family still remains obscure, CD40 has proven its importance (3).
A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. This molecule as well as the other ligands of the family share the property of co-stimulation of T-cell proliferation and are all expressed by activated T-cells.
The programmed cell death has been suggested to be involved in clonal elimination of self-reactive lymphocytes for the normal function of the immune system. Interaction with membrane bound self antigens may eliminate self-reactive nature B cells by apoptosis. Antigen-receptor mediated B cell apoptosis is blocked when a signal is transduced via the CD40 molecule on the B cell surface (10).
Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T-helper cells. Thus the CD40 - CD40L interaction plays a central role in the various phases of the B cell response to T-dependent antigens (8).
Taken together, B cells can participate in regulating their own destruction. Protection against Fas-dependent apoptosis afforded by immunoglobulin-receptor engagement may constitute a fail-safe mechanism that eliminates bystander B cells activated by CD40L - expressing T cells, but ensures survival of antigen-specific B cells (9).
CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40 (4, 6).
CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome (1) is targeted to the CD40L gene, it is functional involved in B cell hybridomas (5) and chronic lymphocytic leukemia (2) as well as several autoimmune diseases (7).
Sodium azide is a poisonous and hazardous substance, handle and dispose of properly
Version 8 - 05.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.