Anti-Human CD40 Azide Free
Product Specifications
- Catalogue N°
- 854.280.000 - 200µg / 200µl
854.280.005 - 500µg / 500µl - Target species
- Human
- Specificity
- Recognises the Bp50 antigen, a 48 kDa protein
- Clone
- B-B20
- Application
- Flow Cytometry
- Immunohistochemistry
- Functional assay
- Hybridoma
- Myeloma X63/AG.8653 x Balb/c spleen cells
- Immunisation
- Raji cell line
- Quantity
- 200µg or 500µg (Discovery Size also available please enquire)
- Isotype
- Mouse IgG1 Kappa light chain
- Format
- Phosphate-buffered saline. Sterile-filtered through 0.22 µm. Carrier and preservative free
- Workshop
- VI
- Storage
- Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
- Biological Activity
- Stimulates B-cell proliferation together with IL-4
References
- Cagigi, A. et al., PLoS One,2009; 4(5): e5427. - Pubmed link
- De Goer de Herve M-G. et al., Blood, 2005; 106(8) : 2806-14 - Pubmed link
- Durali D. et al., Blood, 2003; 102(12) : 4084-4089 - Pubmed link
- Jeannin, P. et al., J Biol Chem,1997; 272(25): 15613-9. - Pubmed link
- Kap, Y. S. et al., Journal of Histochemistry & Cytochemistry,2009;57(12):1159-1167. - Pubmed link
- O'Neill, A. M. et al.,Cancer Biol Ther.,2011; 11(7): 651-8 - Pubmed link
- Puig-Kröger A. et al., Blood, 2001;98(7):2175-2182 - Pubmed link
- Vermot-Desroches, C. et al., Immunol Lett.,1995; 48(1): 1-9. - Pubmed link
- Wassmer, S. C. et al., Cell Microbiol.,2011;13(2): 198-209 - Pubmed link
- Wassmer, S. C. et al., Infect Immun.,2006; 74(1): 645-53. - Pubmed link
- Wassmer, S. C. et al., J Immunol.,2006; 176(2): 1180-4. - Pubmed link
- Wassmer, S. C. et al.,J Infect Dis.,2004;189(2):180-9 - Pubmed link
BACKGROUND
CD40 belongs to the TNF receptor superfamily. While the biological role of some of the ligand-receptor pairs in this family still remains obscure, CD40 has proven its importance (3).
A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. This molecule as well as the other ligands of the family share the property of co-stimulation of T-cell proliferation and are all expressed by activated T-cells.
The programmed cell death has been suggested to be involved in clonal elimination of self-reactive lymphocytes for the normal function of the immune system. Interaction with membrane bound self antigens may eliminate self-reactive nature B cells by apoptosis. Antigen-receptor mediated B cell apoptosis is blocked when a signal is transduced via the CD40 molecule on the B cell surface (10).
Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T-helper cells. Thus the CD40 - CD40L interaction plays a central role in the various phases of the B cell response to T-dependent antigens (8).
Taken together, B cells can participate in regulating their own destruction. Protection against Fas-dependent apoptosis afforded by immunoglobulin-receptor engagement may constitute a fail-safe mechanism that eliminates bystander B cells activated by CD40L - expressing T cells, but ensures survival of antigen-specific B cells (9).
CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40 (4, 6).
CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome (1) is targeted to the CD40L gene, it is functional involved in B cell hybridomas (5) and chronic lymphocytic leukemia (2) as well as several autoimmune diseases (7).
Version 12 - 05.19
For research use only
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