- Catalogue N°
- 869.040.010 - 10 x 96 tests
- Target species
- Recognizes natural human IL-12. Specific to p70 heterodimer.
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISpot matched antibody pairs are extensively validated and include pre-titrated capture antibody and biotinylated detection antibody. Antibodies are supplied in quantities sufficient for 10 x 96 samples.
IL-12 is a potent regulator of cell mediated immune response produced by activated monocytes / macrophages cells, B lymphocytes and connective tissue type mast cells.
IL-12p70 is the biologically active form and is composed of two subunits: IL-12p35 (also named IL-12A) and IL-12p40 (also named IL-12B). The p35 subunit has homology to IL-6, while p40 has homology with IL-23. The p40 subunit has been found to be expressed in a higher excess over p70. It is present in serum and plasma as a monomer, as an heterodimer with IL-12p35 (biologically active IL-12p70) or a heterodimer with IL-23p19 (IL-23). The subunits are genetically unrelated and are regulated independently: IL-12p40 is produced constitutively and in excess of IL-12p35 and IL-23p19.
IL-12 has been found to bind to IL-12R. IL-12R has been reported to be present on IL-2 activated CD4+, CD8+ and CD56+ cells. IL-12 exerts a variety of biological effects on human T and NK cells. IL-12 induces an IFNg production and other cytokines from peripheral blood T and NK cells. Its role is directing development and proliferation of Th1 cells. Thus IL-12 is linked with autoimmunity, high level have also been reported for chronic inflammatory reactions, bacterial and viral infection.
IL-12 is a pleiotropic cytokine initially also called cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF) mainly produced by monocytes, macrophages and dendritic cells in response to bacterial products or upon interaction with activated T Cell.
IL-12 induces IFNg production and increases proliferation and cytotoxic activity of T and NK cells. Moreover, IL-12 induces CD4+ polarization to the Th1 phenotype that mediates immunity against intracellular pathogens.
Several studies show that IL-12p40, in monomer or homodimer form, was able to bind to IL-12R beta-1 and seem to be antagonist to IL-12p70 and IL-23 bounding.
Version 5 - 05.20
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