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Murine IL-12 ELISA Kit

Product Specifications

Catalogue N°
660.040.096 - 1 x 96 (pre-coated)
660.040.192 - 2 x 96 (pre-coated)
Assay Range
31.25 pg/ml - 2000 pg/ml
Sensitivity
5.9 pg/ml
Target species
Murine
Specificity
Recognizes both natural and recombinant murine IL-12
Incubation
3h10
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
10 µl
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
mIL-12 ELISA Kit
mIL-12 ELISA Kit

BACKGROUND

Interleukin-12 (IL-12) is a pleiotropic cytokine, formerly termed cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF), which is produced primarily by stimulated macrophages. IL-12 has been shown to be a proinflammatory cytokine produced by phagocytic cells, B cells, and other antigen - presenting cells that modulate adaptive immune responses by favoring the generation of T-helper type 1 cells.

IL-12 exerts a variety of biological effects on T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of interferon (IFN) gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-gamma then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN-gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections.

Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.

Version 3 - 07.19

For research use only

Documents

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Formats Available

660.040.096 1 x 96 (pre-coated)
660.040.192 2 x 96 (pre-coated)

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