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Product Specifications

Catalogue N°
865.000.048 - 1 x 48 Discovery (pre-coated)
865.000.096 - 1 x 96 (pre-coated)
865.000.192 - 2 x 96 (pre-coated)
Assay Range
31.25 pg/ml - 1000 pg/ml
15 pg/ml
Target species
Recognizes both natural and recombinant rat TNF-a
Sample Type
Serum, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other rat cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Example of Rat TNF-a Standard Curve


Tumor Necrosis Factor (TNFa), also known as cachectin, is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNFa circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNFa also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNFa. Due to its varied actions throughout the immune system, TNFa may play a role in the pathogenesis of many disease states.

TNFa production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNFa within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNFa is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNFa is enhanced by gamma interferon. TNFa then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1. Recent evidence has suggested an intracellular role for this peptide.

Version 23 - 02.22

For research use only


For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

865.000.048 1 x 48 Discovery (pre-coated)
865.000.096 1 x 96 (pre-coated)
865.000.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Rat TNF-α ELISA Kit 865.000.048 - 1 x 48 Discovery (pre-coated) 865.000.096 - 1 x 96 (pre-coated) 865.000.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders