Human RANTES ELISA kit
Product Specifications
- Catalogue N°
- 873.110.048 - 1 x 48 Discovery (pre-coated)
873.110.096 - 1 x 96 (pre-coated)
873.110.192 - 2 x 96 (pre-coated) - Assay Range
- 250 – 6.25 pg/ml
- Sensitivity
- 1.2 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant RANTES
- Incubation
- 1h45
- Sample Type
- Cell culture supernatant, serum and plasma
- Sample Size
- 10 µl
- Cross Reaction
- No cross reactivity with other cytokines tested
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
- Synonym
- CCL5
- C-C motif chemokine ligand 5
BACKGROUND
CCL5, also known as Regulated upon Activation, Normal T Cell Expressed and Secreted (RANTES), is a member of the CC family of chemokine (Chemotactic cytokines that control the migration of immune cells in tissue). The mature form of the protein consists of 68 amino acids with a molecular mas of approx 8 kDa.
Mainly expressed by T-cells and monocytes and abundantly by epithelial cells, fibroblasts, and thrombocytes, it plays an active role in recruiting leucocytes into inflammatory sites through receptors CCR1, CCR3, CCR4 and CCR5 and inducing their proliferation and activation.
Described as the major HIV-1 co-receptor, it inhibits the virus entry and replication within cells at low concentration. The CCR5-CCL5 blockade maybe thus an effective strategy for HIV prevention.
RANTES is involved in transplantations (level is higher during rejection of renal transplant) and plays an important role in various human disorders, such as atherosclerosis, COVID-19, SARS, atopic dermatitis, asthma, glomerulonephritis, alcohol liver disease, acute liver failure and viral hepatitis. Increased level of RANTES was discovered in lots of cancer, hepatocellular carcinoma, stomach, prostate and pancreatic cancer).
Version 1 - 11.22
For research use only
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