Human Granzyme B EasySplit ELISpot Kit

Product Specifications

Catalogue N°

856.131.001PCS - 1 x 96 Discovery (pre-coated strip plate)
856.131.002PCS - 2 x 96 (pre-coated strip plates)
856.131.005PCS - 5 x 96 (pre-coated strip plates)

Target species

Human

Specificity

Recognizes natural human Granzyme B

Incubation

3h after cell stimulation

Kit Content

Diaclone EasySplit ELISpot kits include: 
Pre-coated strip plate
Detection biotinylated antibody
Streptavidin Alkaline Phosphatase Conjugate
Bovine Serum Albumin
Ready-to-use BCIP/NBT Substrate Buffer

• References
• Related products

BACKGROUND

Granzyme B belongs to the granzymes family, which are exogenous serine proteinases. Granzymes are produced by cytotoxic lymphocytes (CTLs) and NK cells.

Activated cytotoxic T lymphocytes and NK cells are important effectors in immunologic defenses against tumor cell proliferation, viral infection and transplant tissue. They induce apoptosis via multiple mechanism thanks to granules exocytosis. Following adhesion of the CTL on the target cell (via CTL-receptor and antigen-presenting MHC molecules), CTLs secrete cytoplasmic granules containing granules and enzymes including granzymes and perforine (pore-forming protein). Granules are then released in the intercellular space, perforine forms a transmembrane pore. Through these pores, granzymes enter the cytosol of the target cell.

Granzyme B is the most powerful pro-apoptotic member of granzyme family. Granzyme B activates the intracellular caspases induising finally the death of the cell.

Granzymes are diagnostics markers for many disorders (viral infection, lymphoma’s and carcinoma’s, auto-immune disease and rejection of transplant).

Version 1 - 07.23

For research use only

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