- Catalogue N°
- 853.810.000 - 200µg / 200µl
853.810.005 - 500µg / 500µl
- Target species
- Recognises the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1), E-selectin, a 97 kDa protein
- Myeloma X63/AG.8653 x Balb/c spleen cells
- Activated human endothelial cells
- 200µg or 500µg (Discovery Size also available please enquire)
- Mouse IgG1 Kappa light chain
- Phosphate-buffered saline. Sterile-filtered through 0.22 µm. Carrier and preservative free
- Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
E-selectin (CD62E) is a 115 kDa glycoprotein, expressed only on endothelial cells after activation with interleukin-1 (IL-1), tumor necrosis factor-α (TNFα), or bacterial lipopolysaccharides. After stimulation of the endothelial cells, the newly synthesized E-selectin is rapidly detected, with maximum surface expression after 3-6 h and return to basal levels within 24 h. This rapid down-regulation, although poorly understood, has been explained by the release of a soluble form of E-selectin and the internalization of the molecule. This regulation of E-selectin expression could be crucial for controlling leukocyte accumulation in inflammatory responses. Several E-selectin ligands have been identified on leukocytes, but have not yet been cloned. Monoclonal antibodies (mAbs) specific for E selectin have been shown to inhibit leukocyte transmigration. It has been suggested that leukocyte binding to E-selectin on activated endothelium positively regulates CD11b (Mac-1) on leukocytes and induces increased adhesion by the ICAM-1 / Mac-1 interaction.
Version 5 - 06.19
For research use only
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