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Anti-Human CD95 Azide Free

Product Specifications

Catalogue N°
853.030.000 - 200µg / 200µl
853.030.005 - 500µg / 500µl
Target species
Human
Specificity
Recognises the Fas, APO1 antigen, a 45 kDa protein
Clone
B-D29
Application
ELISA
Functional assay
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
Recombinant Fas
Quantity
200µg or 500µg (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline. Sterile-filtered through 0.22 μm and treated to remove endotoxins. Carrier and preservative free
Workshop
VI
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Biological Activity
Inhibits FasL induced apoptosis
Synonym
APO-1
FAS

BACKGROUND

Fas (APO-1, CD95) is a transmembrane protein belonging to the TNF receptor family, capable of mediating apoptotic death of transformed cells as well as activated human T cells. The cell death signal can be induced by anti-Fas antibodies or by the Fas (Fas-L) ligand belonging to the TNF family. The in vivo expression of these two Fas and Fas-L molecules is differently regulated since the expression of Fas is pleiotropic whereas Fas-L is rather expressed in immunity sites (testes, ovaries, brain, eye) and by activated T cells. Tumor cells regardless of their tissue origin may also express Fas-L (pathological).

Genetic and immunological studies have shown that the Fas system is on the one hand involved in the process of peripheral clonal deletion allowing the maintenance of self-tolerance, on the other hand that it regulates the amplitude and duration of immune responses. and finally that it represents one of the mechanisms of lysis of the target cells in the antiviral cytotoxic T response.

When Fas-dependent apoptosis can not occur, due to genetic mutations in the Fas or Fas-L genes, a lymphoproliferative syndrome and autoimmune manifestations are observed in humans and mice. Conversely, an exacerbation of the Fas system may be responsible for tissue destruction. For example, the single mouse injection of anti-Fas antibodies induces massive liver destruction and mouse death within a few hours, leading to the hypothesis of Fas involvement in fulminant hepatitis.

Version 7 - 06.19

For research use only

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Formats Available

853.030.000 200µg / 200µl
853.030.005 500µg / 500µl

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