- Catalogue N°
- 670.020.096 - 1 x 96 (pre-coated)
670.020.192 - 2 x 96 (pre-coated)
- Assay Range
- 62.5 à 2000 pg/ml
- 48 pg/ml
- Target species
- Recognizes both natural and recombinant rat TGF-β1
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other rat cytokines
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Transforming growth factor-b (TGF-b) belongs to a family of dimeric 25 kDa polypeptides that are ubiquitously distributed in tissues and synthesized by many different cells. Three isoforms of transforming
Growth Factor-b (TGF-beta1, beta-2 and beta-3) exist in mammals. They play critical roles in growth regulation and development. Each isoform is encoded by a unique gene on different chromosomes. All three of these growth factors are secreted by most cell types, generally in a latent form, requiring activation before they can exert biological activity. The TGFbetas possess three major activities: they inhibit proliferation of most cells, but can stimulate the growth of some mesechymal cells; they exert immunosuppressive effects and they enhance the formation of extracellular matrix. Two types of membrane receptors possessing kinase
activity are involved in signal transduction. The TGF-betas are involved in wound repair processes and in starting inflammatory reaction and then in the resolution through chemotactic attraction of inflammatory cells and fibroblast.
TGF-b1 is the first recognized transforming growth factor, its subunits of each 12.5 kDa are bound via disulphide bridges. TGF-b1 is inhibitive to T- and B cell proliferation as well as to maturation and activation of macrophages. It furthermore inhibits activity of natural killer cells and lymphokine activated killer cells and blocks production of cytokines.
Version 5 - 07.19
For research use only
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