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Murine IL-15 ELISA kit

Product Specifications

Catalogue N°
660.090.096 - 1 x 96 (pre-coated)
660.090.192 - 2 x 96 (pre-coated)
Assay Range
6.25 pg/ml - 400 pg/ml
Sensitivity
2.0 pg/ml
Target species
Murine
Specificity
Recognizes both natural and recombinant murine IL-15
Incubation
3h30
Sample Type
Cell culture supernatant and serum tested, other body fluids suitable
Sample Size
50 µl
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
mIL-15 ELISA Kit
mIL-15 ELISA Kit

BACKGROUND

Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, β and γ, are shared with the IL-2 receptor, while the α subunit is unique to IL-15.

Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15Rα. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the βγ subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by murine dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.

Version 3 - 07.19

For research use only

Documents

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Formats Available

660.090.096 1 x 96 (pre-coated)
660.090.192 2 x 96 (pre-coated)

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