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Murine TGF-β1 ELISA Kit

Product Specifications

Catalogue N°
660.050.096 - 1 x 96 (pre-coated)
660.050.192 - 2 x 96 (pre-coated)
Assay Range
31.3 pg/ml - 2000 pg/ml
Sensitivity
9 pg/ml
Target species
Murine
Specificity
Recognizes both natural and recombinant murine TGF-β1
Incubation
5h30
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other murine cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
TGF-b1
TGF-beta1
mTGF-b1 ELISA Kit
mTGF-b1 ELISA Kit

BACKGROUND

Transforming growth factor-b (TGF-b) belongs to a family of dimeric 25 kDa polypeptides that are ubiquitously distributed in tissues and synthesized by many different cells.Three isoforms of transforming Growth Factor-b (TGF-beta1, beta-2 and beta-3) exist in mammals. They play critical roles in growth regulation and development. Each isoform is encoded by a unique gene on different chromosomes. All three of these growth factors are secreted by most cell types, generally in a latent form, requiring activation before they can exert biological activity. The TGF-betas possess three major activities: they inhibit proliferation of most cells, but can stimulate the growth of some mesechymal cells; they exert immunosuppressive effects and they enhance the formation of extracellular matrix. Two types of membrane receptors possessing kinase activity are involved in signal transduction. The TGF-betas are involved in wound repair processes and in starting inflammatory reaction and then in the resolution through chemotactic attraction of inflammatory cells and fibroblast.

TGF-b1 is the first recognized transforming growth factor, its subunits of each 12.5 kDa are bound via disulphide bridges. TGF-b1 is inhibitive to T- and B cell proliferation as well as to maturation and activation of macrophages. It furthermore inhibits activity of natural killer cells and lymphokine activated killer cells and blocks production of cytokines.

Version 4 - 07.19

For research use only

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Formats Available

660.050.096 1 x 96 (pre-coated)
660.050.192 2 x 96 (pre-coated)

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