- Catalogue N°
- 670.100.096 - 1 x 96 (pre-coated)
670.100.192 - 2 x 96 (pre-coated)
- Assay Range
- 62.5 pg/ml - 2000 pg/ml
- Target species
- Recognizes both natural and recombinant rat IL-2
- Sample Type
- Cell culture supernatant and serum tested, other body fluids suitable
- Sample Size
- 50 µl
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Interleukin-2 (IL-2) plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response.
IL-2 gene expression is regulated at the transcriptional level by several activation pathways. Antigen-specific proliferation of helper and cytotoxic T-lymphocytes following stimulation is critically dependent on IL-2 expression, secretion, and binding to receptors for IL-2 induced in an autocrine fashion on the surface of T-cells.
Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon-g and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation.
Alterations in the ability of T-cells to synthesize IL-2 have been observed in physiologic and pathologic states.
Version 3 - 07.19
For research use only
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