Murine IFN-γ ELISA Kit
Product Specifications
- Catalogue N°
- 860.050.048 - 1 x 48 Discovery (pre-coated)
860.050.096 - 1 x 96 (pre-coated)
860.050.192 - 2 x 96 (pre-coated) - Assay Range
- 31.25 pg/ml - 1000 pg/ml
- Sensitivity
- 15 pg/ml
- Target species
- Murine
- Specificity
- Recognizes both natural and recombinant murine IFN-g
- Incubation
- 3h45
- Sample Type
- Serum, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other murine cytokines
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
- Synonym
- IFN-g
- IFN-gamma
References
- Carrion, J. et al.,PLoS Negl Trop Dis.,2013; 7(2): e2077 - Pubmed link
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- El-Shennawy, A. M. et al.,MedGenMed,2007; 9(1): 15 - Pubmed link
- Garcia-Perez, M. A. et al., Hum Reprod.,2006;21(4):880-7 - Pubmed link
- Gil, O. et al., Clin. Vaccine Immunol.,2008;15(11):1742-1744. - Pubmed link
- Iborra, S. et al., Blood,2011; 117(19): 5102-5111 - Pubmed link
- Iborra, S. et al., Clin Exp Immunol.,2007; 150(2): 375-85. - Pubmed link
- Iborra, S. et al., Infect Immun.,2005;73(9): 5842-52. - Pubmed link
- Kaur, R. et al., J Tradit Complement Med. 2017 Jun 27;8(1):251-260 - Pubmed link
- Li Xiangming et al., Int. Immunol., 2005; 17(10):1293-302 - Pubmed link
- Matsumura, S. et al., J. Immunol.,2008; 181(5): 3099-3107. - Pubmed link
- Newcomb, E. W. et al.,Radiat Res.,2010; 173(4): 426-32 - Pubmed link
- Pasricha, R. et al.,Pathog Dis.,2014; 72(3): 188-196 - Pubmed link
- Plant, L. et al., Infect Immun.,2006; 74(6): 3538-46. - Pubmed link
- Ramirez, L. et al., J Biomed Biotechnol., 2010: 181690 - Pubmed link
- Sharma, M. et al., PLoS Negl Trop Dis.,2012; 6(5): e1629 - Pubmed link
- Smith, V. P. et al., J Gen Virol.,2000; 81(Pt 5): 1223-30. - Pubmed link
- Zhang, Q. et al., Oncol Lett.,2016; 12(6): 4767-4772. - Pubmed link
- Zhu, M. et al., J Med Microbiol.,2003; 52(Pt 3): 223-8. - Pubmed link
- Compte,M. et al.,Nat Commun. 2018 Nov 15;9(1):4809. - Pubmed link
- Einarsdottir, BO. et al., Cell Death Dis. 2018 Jul 24;9(8) :810 - Pubmed link
- Mikkelsen, K. et al.,Front Immunol. 2019 Jul 31;10:1791. - Pubmed link
- Castejón, M. L. et al., Nutrients. 2021 Feb; 13(2): 311. - Pubmed link
- García-Bernal, D. et al., J Cell Mol Med. 2020 Jul; 24(14): 8031–8044. - Pubmed link
- Santos, F. et al., Microb Cell Fact. 2020; 19: 42. - Pubmed link
- Kaur, R., & Kaur, S., J Ayurveda Integr Med. 2020 Jan-Mar; 11(1): 53–61. - Pubmed link
- Montoya, T. et al., Antioxidants (Basel) 2021 May; 10(5): 650. - Pubmed link
- Nazarian, S. et al., Mol Immunol. 2022 Sep; 149: 107–118. - Pubmed link
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BACKGROUND
Different populations of T-cells secrete differing patterns of cytokines that ultimately lead to different immune responses. IFNγ production is a key function of Th1, CD8+ CTLs and also NK cells. It is a cytokine critical for cell mediated immunity against viral and intracellular bacterial infections and is involved in the inflammatory response following secretion via macrophage activation and stimulation of antibody secretion. IFNg is the hallmark effector cytokine of Th1 and therefore is an excellent marker for identifying a host response to intracellular pathogens.
IFNg is produced during infection by T cells of the cytotoxic/suppressor phenotype (CD8) and by a subtype of helper T cells, the Th1 cells. Th1 cells secrete IL-2, IL-3, TNFα and IFNγ, whereas Th2 cells mainly produce IL-3, IL-4, IL-5, and IL-10, but little or no IFNγ. IFNγ preferentially inhibits the proliferation of Th2 but not Th1 cells, indicating that the presence of IFNγ during an immune response will result in the preferential proliferation of Th1 cells.
In addition, IFNγ has several properties related to immunoregulation. IFNγ is a potent activator of mononuclear phagocytes, and activates macrophages to kill tumor cells by releasing reactive oxygen intermediates and TNFα. IFNγ induces or augments the expression of MHC antigens on macrophages, T and B cells and some tumor cell lines. On T and B cells IFNγ promotes differentiation. It enhances proliferation of activated B cells and can act synergistically with IL-2 to increase immunoglobulin light-chain synthesis.
The role of IFNγ as a disease marker has been demonstrated for a number of different pathological situations including, viral infection, autoimmune disease, transplant rejection, diabetes and allergy.
Version 17 - 09.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.