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Murine GM-CSF ELISA kit

Product Specifications

Catalogue N°
860.060.048 - 1 x 48 Discovery (pre-coated)
860.060.096 - 1 x 96 (pre-coated)
860.060.192 - 2 x 96 (pre-coated)
Assay Range
15.6 pg/ml - 500 pg/ml
Sensitivity
9 pg/ml
Target species
Murine
Specificity
Recognizes both natural and recombinant murine GM-CSF
Incubation
3h45
Sample Type
Serum, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other murine cytokines
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
mGM-CSF ELISA Kit
Example of Murine GM-CSF Standard Curve

BACKGROUND

Granulocyte - macrophage colony - stimulating factor (GM-CSF) is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes. GM-CSF can be produced by a wide variety of tissue types, including fibroblasts, endothelial cells, T-cells, macrophages, mesothelial cells, epithelial cells and many types of tumor cells. In most of these tissues, inflammatory mediators, such as interleukin-1, interleukin-6, tumor necrosis factor or endotoxin, are patent inducers of GM-CSF gene expression.

The mGM-CSF is a glycoprotein of 96 aa sharing 54% sequence identity with human GM-CSF.

The biological effects of GM-CSF are mediated through binding to cell surface receptors, which appear to be widely expressed by hematopoietic cells and also by some non-hematopoietic cells, such as endothelial cells. At least two different functional classes of GM-CSF receptor have been identified. The neutrophil GM-CSF receptor exclusively binds GM-CSF, while interleukin-3 competes for binding of GM-CSF to a second class of receptors detected on some leukemic cell lines. Monitoring of GM-CSF has been found relevant in human prostate cancer, severe mucositis, AIDS, bone marrow transplantation, renal cell carcinoma and prostate cancer, acute lymphoblastic leukemia, pulmonary inflammation, hematological malignancies, infection, lung cancer, crohn’s disease.

Version 7 - 11.21

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

860.060.048 1 x 48 Discovery (pre-coated)
860.060.096 1 x 96 (pre-coated)
860.060.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Murine GM-CSF ELISA kit 860.060.048 - 1 x 48 Discovery (pre-coated) 860.060.096 - 1 x 96 (pre-coated) 860.060.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders