Human MICA ELISA Kit
Product Specifications
- Catalogue N°
- 850.910.048 - 1 x 48 Discovery (pre-coated)
850.910.096 - 1 x 96 (pre-coated)
850.910.192 - 2 x 96 (pre-coated) - Assay Range
- 156.25 pg/ml - 5000 pg/ml
- Sensitivity
- 123 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human MICA
- Incubation
- 3h45
- Sample Type
- Supernatant, Buffered Solutions. Not suitable for use with serum and plasma.
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with IL-7, IL-12p40, IL-2R, Trail, CD31, IL-13Rα2, IL-5, gp130, TNFα.
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
References
- Bedel, R. et al., Cancer Res.,2011;71(5): 1615-1626 - Pubmed link
- Goto, K. et al., Sci Rep.,2016; 6: 38407 - Pubmed link
- Goto, K. et al.,Oncotarget. 2018 Apr 10;9(27):18821-18831. - Pubmed link
- Arai, J. et al., Cancer Immunol Immunother. 2021; 70(1): 203–213. - Pubmed link
- Arai, J. et al., BMC Cancer. 2022; 22: 428. - Pubmed link
BACKGROUND
MICA is a transmembrane glycoprotein that functions as a ligand for human NKG2D, an activating receptor expressed on NK Cells, NKT Cells, dg T-cells and CD8+ba T-cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effectors cells. MICA recognition is involved in tumour surveillance, viral infections, and autoimmune diseases.
Major histocompatibility complex (MHC) class I chain-related gene A and B (MICA and MICB) are transmembrane glycoproteins that function as ligand for NKG2D. These two proteins possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with b2-microglobulin. The genes encoding MICA/B are found within the MHC on human chromosome 6.
MICA and MICB have no role in antigen presentation but function as signal of cellular distress and interact with NKG2D-DAP10, the activating receptor. They are frequently expressed in epithelial tumour and may promote anti tumour NK and T-cell response.
Intestinal cells express MICA/MICB which are up-regulated under stress and in many gastrointestinal tumours. Release of MIC molecules from the cell surface is thought to constitute in immune escape mechanism of tumour cells.
MICA/MICB expression is elevated in the sera of patients with colorectal carcinoma and widely expressed in prostate carcinoma : MICA/MICB may be a novel biomarker for prostate cancer and its expression is also used as a monitor in Crohn’s disease.
Version 14 - 09.22
For research use only
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