Human MCP-1 ELISA kit
Product Specifications
- Catalogue N°
- 873.030.048 - 1 x 48 Discovery (pre-coated)
873.030.096 - 1 x 96 (pre-coated)
873.030.192 - 2 x 96 (pre-coated) - Assay Range
- 31.25 pg/ml - 1000 pg/ml
- Sensitivity
- 5.8pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human MCP-1
- Incubation
- 2h15
- Sample Type
- Serum, plasma, cell culture supernatant, buffered solutions and other body fluids.
- Sample Size
- 100 µl (50µl for serum/plasma)
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
- Synonym
- CCL2
- SCYA-2
- JE
References
- Chavushyan, A. et al., Schizophr Res Treatment,2013: 125264 - Pubmed link
- Hidalgo, M. et al.,Genes Nutr.,2012; 7(2): 295-306 - Pubmed link
- Lu, C. et al., Lupus, 2015;24(1):18-24 - Pubmed link
- Mazaherioun, M. et al.,Med Princ Pract. 2017;26(6):535-541. - Pubmed link
- Romacho, T. et al.,Nutrients,2015; 7(2): 865-86 - Pubmed link
- Sobjanek, M. et al., Postepy Dermatol Alergol.,2016;33(5): 381-385. - Pubmed link
- Estévez-Santiago, R. et al.,Nutrients. 2019 Jul 5;11(7). pii: E1533 - Pubmed link
- Bautista-Expósito, S. et al., Antioxidants (Basel) 2020 Oct; 9(10): 984. - Pubmed link
- Modgil, S. et al., J Neurosci Rural Pract. 2020 Oct; 11(4): 573–580. - Pubmed link
- Tinelli, F. et al., Int J Mol Sci. 2020 Aug; 21(16): 5763. - Pubmed link
- Szeremeta, A. et al., J Clin Med. 2022 Jul; 11(14): 4213. - Pubmed link
- Kocak-Oztug, N.A. et al., Biomed Res Int. 2022; 2022: 2603287. - Pubmed link
- Ługowska-Umer, H. et al., Postepy Dermatol Alergol. 2021 Dec; 38(6): 1039–1043. - Pubmed link
BACKGROUND
Chemokines (chemoattractant cytokines) represent a superfamily of small secreted proteins that function as intercellular messengers to control migration and activation of leukocytes involved in inflammatory reactions and immunity. Furthermore, chemokines are important mediators of many pathologies such as allergic responses, chronic inflammatory and autoimmune diseases, tumor growth and hematopoietic development.
The CC-chemokine monocyte chemoattractant protein 1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells.
The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-a and IL-1b, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells.
Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses.Elevated levels of MCP-1 have also been found in connection with osseus inflammation and Alzheimer's disease (AD) as well as Myocardial Ischemia and viral infections.
In acute and chronic-active multiple sclerosis (MS) lesions immunoreactivity for MCP-1 was increased whereas MCP-1 was found to be significantly reduced in cerebrospinal fluids (CSF) and chronic lesions of patients with MS. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.
Version 12 - 09.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.