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Human MCP-1 ELISA kit

Product Specifications

Catalogue N°
873.030.048 - 1 x 48 Discovery (pre-coated)
873.030.096 - 1 x 96 (pre-coated)
873.030.192 - 2 x 96 (pre-coated)
Assay Range
31.25 pg/ml - 1000 pg/ml
Sensitivity
5.8pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human MCP-1
Incubation
2h15
Sample Type
Serum, plasma, cell culture supernatant, buffered solutions and other body fluids.
Sample Size
100 µl (50µl for serum/plasma)
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
CCL2
SCYA-2
JE
MCP-1 ELISA Kit
Example of Human MCP-1 Standard Curve

BACKGROUND

Chemokines (chemoattractant cytokines) represent a superfamily of small secreted proteins that function as intercellular messengers to control migration and activation of leukocytes involved in inflammatory reactions and immunity. Furthermore, chemokines are important mediators of many pathologies such as allergic responses, chronic inflammatory and autoimmune diseases, tumor growth and hematopoietic development.

The CC-chemokine monocyte chemoattractant protein 1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) was characterized as a monocyte-specific chemoattractant that was later shown to attract also T lymphocytes and NK cells.

The human mature MCP-1 protein consists of 76 amino acids, derived by cleavage of a hydrophobic signal peptide from the 99 aa precursor protein. MCP-1 is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-a and IL-1b, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells.

Because of its target cell specificity, MCP-1 was postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses.Elevated levels of MCP-1 have also been found in connection with osseus inflammation and Alzheimer's disease (AD) as well as Myocardial Ischemia and viral infections.

In acute and chronic-active multiple sclerosis (MS) lesions immunoreactivity for MCP-1 was increased whereas MCP-1 was found to be significantly reduced in cerebrospinal fluids (CSF) and chronic lesions of patients with MS. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally it has been shown to chemoattract CD4+ and CD8+ T lymphocytes, and expression of the MCP-1 chemokine may affect HIV infection via signaling through the CCR2 receptor.

Version 12 - 09.22

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

873.030.048 1 x 48 Discovery (pre-coated)
873.030.096 1 x 96 (pre-coated)
873.030.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human MCP-1 ELISA kit 873.030.048 - 1 x 48 Discovery (pre-coated) 873.030.096 - 1 x 96 (pre-coated) 873.030.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders