Human IL-13 ELISA Set
Product Specifications
- Catalogue N°
- 851.630.001 - 1 x 96 Discovery
851.630.005 - 5 x 96
851.630.010 - 10 x 96
851.630.015 - 15 x 96
851.630.020 - 20 x 96 - Assay Range
- 3.12 pg/ml - 100 pg/ml
- Sensitivity
- 1.5 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human IL-13
- Incubation
- From sample to end 2h15
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
References
- Arnaud, V. et al., J Infect Dis.,2008;198(3): 418-26. - Pubmed link
- Bredo, G. et al.,Immun Inflamm Dis.,2015; 3(4): 455-68 - Pubmed link
- Ghannam, S. et al., J. Immunol.,2010; 185(1): 302-312 - Pubmed link
- Malmstrom, K. et al.,Clin Transl Allergy ,2014;4:35 - Pubmed link
- Ochensberger, B. et al., Blood,1996; 88(8): 3028-37. - Pubmed link
- Ota M. O. C. et al., J Trop Pediatr, 2006; 52(2):136-40 - Pubmed link
- Ota M. O. C. et al., J. Immunol., 2002; 168(2) : 919 - 925 - Pubmed link
- Pecaric-Petkovic, T. et al., Blood,2009; 113(7): 1526-1534. - Pubmed link
- Schramm G. et al., J. Biol. Chem., 2003; 278(20): 18384 - 18392 - Pubmed link
- Tschopp, C. M. et al., Blood,2006; 108(7): 2290-9 - Pubmed link
- Vekemans J. et al., Infect. Immun., 2004; 72(1): 381 - 388 - Pubmed link
Related products
- 855.140.000 - Anti-Human IL-13 Azide Free
- 879.080.001 - Anti-Human IL-13 Capture Antibody
- 879.080.002 - Anti-Human IL-13 Detection Antibody
- 850.080.048 - Human IL-13 ELISA kit
- 856.121.001 - Human IL-13 ELISpot Set
- 856.121.001PC - Human IL-13 ELISpot Kit
- 869.120.010 - Human IL-13 ELISpot Pair
- 660.080.096 - Murine IL-13 ELISA kit
- 853.750.000 - Anti-Human IL-13 Azide Free
- 858.000.005 - Accessory Pack for ELISA set
BACKGROUND
Interleukin-13 (IL-13) is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. Although the homology between IL-13 and IL-4 at the amino acid level is low, the IL-13 structure determined by NMR is very similar to that of IL-4. Both cytokines share their receptors and signal pathways, giving these two cytokines similar biological properties.
IL-13 is an immunoregulatory cytokine. It has become evident that IL-13 is a key mediator in the pathogenesis of allergic inflammation. IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2.
IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation.
The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models. Interleukin-13 further plays a major role in various other inflammatory diseases including cancer. The IL-13Ralpha2 but not IL-13Ralpha1 chain binds IL-13 with high affinity and is over expressed in a variety of human cancer cells derived from glioma, squamous cell carcinoma of head and neck, and AIDS-associated Kaposi's sarcoma.
Version 11 - 07.19
For research use only
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