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Human IL-13 ELISA Set

Product Specifications

Catalogue N°
851.630.001 - 1 x 96 Discovery
851.630.005 - 5 x 96
851.630.010 - 10 x 96
851.630.015 - 15 x 96
851.630.020 - 20 x 96
Assay Range
3.12 pg/ml - 100 pg/ml
Sensitivity
1.5 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human IL-13
Incubation
From sample to end 2h15
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines
Kit Content
Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

BACKGROUND

Interleukin-13 (IL-13) is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. Although the homology between IL-13 and IL-4 at the amino acid level is low, the IL-13 structure determined by NMR is very similar to that of IL-4. Both cytokines share their receptors and signal pathways, giving these two cytokines similar biological properties.

IL-13 is an immunoregulatory cytokine. It has become evident that IL-13 is a key mediator in the pathogenesis of allergic inflammation. IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2.

IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation.

The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models. Interleukin-13 further plays a major role in various other inflammatory diseases including cancer. The IL-13Ralpha2 but not IL-13Ralpha1 chain binds IL-13 with high affinity and is over expressed in a variety of human cancer cells derived from glioma, squamous cell carcinoma of head and neck, and AIDS-associated Kaposi's sarcoma.

Version 11 - 07.19

For research use only

Documents

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Formats Available

851.630.001 1 x 96 Discovery
851.630.005 5 x 96
851.630.010 10 x 96
851.630.015 15 x 96
851.630.020 20 x 96

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human IL-13 ELISA Set 851.630.001 - 1 x 96 Discovery 851.630.005 - 5 x 96 851.630.010 - 10 x 96 851.630.015 - 15 x 96 851.630.020 - 20 x 96 )

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