Human IL-13 ELISA kit
Product Specifications
- Catalogue N°
- 850.080.048 - 1 x 48 Discovery (pre-coated)
850.080.096 - 1 x 96 (pre-coated)
850.080.192 - 2 x 96 (pre-coated) - Assay Range
- 3.125 pg/ml - 100 pg/ml
- Sensitivity
- 1.5 pg/ml
- Target species
- Human
- Specificity
- Recognizes both natural and recombinant human IL-13
- Incubation
- 2h15
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
References
- Abbal C. et al., Int. Immunol., 1999; 11(9) : 1451 - 1462 - Pubmed link
- Antczak, A. et al., International Journal of Immunopathology and Pharmacology,2016: 0394632015623794 - Pubmed link
- Cameron, L. et al., J Immunol.,2006;177(12): 8633-42. - Pubmed link
- de Nadai, P. et al., J Immunol.,2006;176(10): 6286-93. - Pubmed link
- Fahy O. et al., J. immunol., 2002; 168(11) : 5912 - 5919 - Pubmed link
- Finan, C. et al., PLoS One,2008; 3(10): e3485 - Pubmed link
- Gafvelin, G. et al., J Biol Chem., 2007; 282(6): 3778-87. - Pubmed link
- Halonen, M. et al., J. Immunol.,2009;182(5): 3285-3293. - Pubmed link
- Ismaili, J. et al., Clin Exp Immunol.,2003;133(3):414-21 - Pubmed link
- Leung S. et al., J. Biomol Screen, 2005; 10(2):157 - 167 - Pubmed link
- Pene, J. et al., J. Immunol.,2008; 180(11): 7423-7430. - Pubmed link
- Perrin, Y. et al.,Clin Transl Allergy,2014; 4(1): 1 - Pubmed link
- Piccoli, L. et al.,Clin Dev Immunol., 2012: 483935 - Pubmed link
- Pitta, M. G. et al., J Clin Invest.,2009; 119(8): 2379-87 - Pubmed link
- Saverino D. et al., J. immunol., 2002; 168(1): 207 - 215 - Pubmed link
- Schmitt, C. et al., J Leukoc Biol.,2000; 68(6): 836-44. - Pubmed link
- Tsvetkova-Vicheva, V. M. et al.,Clin Transl Allergy,2014; 4(1): 3 - Pubmed link
- Vekemans, J. et al.,Clin Exp Immunol.,2002;127(3):495-8 - Pubmed link
- Woerly, G. et al., J Leukoc Biol.,2002; 72(4): 769-79. - Pubmed link
- Knuhr, K. et al., Front Immunol. 2018 Oct 10;9:2293 - Pubmed link
- Malhotra, N. et al., Lung India. 2020 Jul-Aug; 37(4): 310–315. - Pubmed link
- Qurashi, T.A. et al., Allergy Asthma Clin Immunol. 2021; 17(1): 119. - Pubmed link
- Sarbinowska, J. et al., Biomolecules. 2021 Jun; 11(6): 890. - Pubmed link
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BACKGROUND
Interleukin-13 (IL-13) is a Th2-type cytokine, secreted from CD4(+) T cells, mast cells, basophils and eosinophils. The IL-13 gene generates a cluster with other Th2-type cytokines such as IL-4 and IL-5. Although the homology between IL-13 and IL-4 at the amino acid level is low, the IL-13 structure determined by NMR is very similar to that of IL-4. Both cytokines share their receptors and signal pathways, giving these two cytokines similar biological properties.
IL-13 is an immunoregulatory cytokine. It has become evident that IL-13 is a key mediator in the pathogenesis of allergic inflammation. IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ralpha and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2.
IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells. However, functional IL-13 receptors have not been demonstrated on human or mouse T cells. Thus unlike IL-4, IL-13 does not appear to be important in the initial differentiation of CD4 T cells into T(H)2-type cells but rather appears to be important in the effector phase of allergic inflammation.
The important role of IL-13 in the pathogenesis of bronchial asthma as well as other allergic diseases has been recognized, based mainly on analyses of mouse models. Interleukin-13 further plays a major role in various other inflammatory diseases including cancer. The IL-13Ralpha2 but not IL-13Ralpha1 chain binds IL-13 with high affinity and is over expressed in a variety of human cancer cells derived from glioma, squamous cell carcinoma of head and neck, and AIDS-associated Kaposi's sarcoma.
Version 16 - 02.22
For research use only
For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.