- Catalogue N°
- 851.880.001 - 1 x 96 Discovery
851.880.005 - 5 x 96
851.880.010 - 10 x 96
851.880.015 - 15 x 96
851.880.020 - 20 x 96
- Assay Range
- 62.5 pg/ml - 2000 pg/ml
- 20 pg/ml (determined with Diaclone ELISA kit)
- Target species
- Recognizes the p40 monomer, dimer or heterodimer (p40+p35) forms of the molecule
- From sample to end 1h45
- Sample Type
- Serum, Plasma, Cell culture supernatant, buffered solution
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
- p40 + p35
IL-12 is a pleiotropic cytokine initially called cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF) mainly produced by monocytes, macrophages and dendritic cells in response to bacterial products or upon interaction with activated T Cell.
IL-12 induces IFNg production and increases proliferation and cytotoxic activity of T and NK cells. Moreover, IL-12 induces CD4+ polarization to the Th1 phenotype that mediates immunity against intracellular pathogens.
IL-12p70 is the biologically active form and is composed of two subunits: IL-12p35 (also named IL-12A) and IL-12p40 (also named IL-12B). The p35 subunit has homology to IL-6, while p40 has homology with IL-23. The p40 subunit has been found to be expressed in a higher excess over p70. It is present in serum and plasma as a monomer, a heterodimer with IL-12p35 (biologically active IL-12p70) or heterodimer with IL-23p19 (IL-23). The subunits are genetically unrelated and are regulated independently: IL-12p40 is produced constitutively and in excess of IL-12p35 and IL-23p19.
Several studies show that IL-12p40, in monomer or homodimer form, was able to bind to IL-12R beta-1 and seem to be antagonist to IL-12p70 and IL-23 bounding.
Following these properties and the role of IL-12 in autoimmunity, IL-12p40 was described as a target in the treatment of autoimmune and systemic inflammatory diseases (Crohn’s disease, Psoriasis, multiple sclerosis).
Version 4 - 07.19
For research use only
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