- Catalogue N°
- 851.550.001 - 1 x 96 Discovery
851.550.005 - 5 x 96
851.550.010 - 10 x 96
851.550.015 - 15 x 96
851.550.020 - 20 x 96
- Assay Range
- 6.25 pg/ml - 200 pg/ml
- 3 pg/ml
- Target species
- Recognizes both natural and recombinant human IL-12. Specific to p70 heterodimer.
- From sample to end 3h45
- Sample Type
- Serum, Plasma, Cell culture supernatant
- Sample Size
- 100 µl
- Cross Reaction
- No cross reactivity with other human cytokines
- Kit Content
- Diaclone ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.
IL-12 is a pleiotropic cytokine initially called cytotoxic lymphocyte maturation factor (CLMF) or natural killer cell stimulatory factor (NKSF). IL-12 is a potent regulator of cell mediated immune response produced by activated monocytes/macrophages cells, dendritics cells, neutrophils, B lymphocytes and connective tissue type mast cells. IL-12 is produced in response to bacterial products, intracellular pathogens, or upon interaction with activated T cells.
The biologically active form of IL-12 is 70 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) light chain and 40 kDa (p40) heavy chain subunits. The p35 subunit has homology to IL-6, while p40 has homology with IL-23. The two subunits are genetically unrelated and are regulated independently: IL-12p40 is produced constitutively and in excess of p35.
The receptor for IL-12p70 is composed of two subunits, IL-12R beta 1 and IL-12R beta 2 which bind p40 and p35 subunits respectively. Through JAKS and STAT4 signaling pathway, IL-12 induces IFNg production and increases proliferation and cytotoxic activity of T and NK cells. Moreover, IL-12 induces CD4+ polarization to the Th1 phenotype that mediates immunity against intracellular pathogens.
Aberrant level of IL-12 in plasma was described in several immune diseases: multiple sclerosis, autoimmune encephalitis, autoimmune subcutaneous lupus erythramatosus and rheumatoid arthritis. High level have also been reported for chronic inflammatory reactions, bacterial and viral infection (mycobacterial, salmonella, HIV).
Following these properties and its role in autoimmunity, IL-12 was described as a target in the treatment of autoimmune and systemic inflammatory diseases (Crohn’s disease, Psoriasis, multiple sclerosis).
Version 9 - 07.19
For research use only
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