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Anti-Human MICA Unconjugated

Product Specifications

Catalogue N°
855.373.020 - 200 tests / 2ml
Target species
Human
Specificity
Recognises both natural and recombinant human MICA
Clone
B-Z26
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
Recombinant human MICA
Quantity
200 Tests / 2ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline with 1 % BSA and 0.09 % sodium azide
Storage
Store at +2-8°C for 12 months. DO NOT FREEZE.
Working Dilution
Use 10 μl to label 106 cells or 100 μl of whole blood
Anti-Human MICA Unconjugated
A typical staining pattern with the B-Z26 monoclonal antibody on U266 cell line

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

MICA is a transmembrane glycoprotein that functions as a ligand for human NKG2D, an activating receptor expressed on NK Cells, NKT Cells, dg T-cells and CD8+ba T-cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effectors cells. MICA recognition is involved in tumour surveillance, viral infections, and autoimmune diseases.

Major histocompatibility complex (MHC) class I chain-related gene A and B (MICA and MICB) are transmembrane glycoproteins that function as ligand for NKG2D. These two proteins possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with b2-microglobulin. The genes encoding MICA/B are found within the MHC on human chromosome 6.

MICA and MICB have no role in antigen presentation but function as signal of cellular distress and interact with NKG2D-DAP10, the activating receptor.They are frequently expressed in epithelial tumour and may promote anti tumour NK and T-cell response.

Intestinal cells express MICA/MICB which are up-regulated under stress and in many gastrointestinal tumours. Release of MIC molecules from the cell surface is thought to constitute in immune escape mechanism of tumour cells.

MICA/MICB expression is elevated in the sera of patients with colorectal carcinoma and widely expressed in prostate carcinoma : MICA/MICB may be a novel biomarker for prostate cancer and its expression is also used as a monitor in Crohn’s disease.

Version 5 - 06.19

For research use only

Documents

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Formats Available

855.373.020 200 tests / 2ml

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Anti-Human MICA Unconjugated 855.373.020 - 200 tests / 2ml )

Please contact us to discuss bulk orders