Search for products

Anti-Human CD141 Unconjugated

Product Specifications

Catalogue N°
854.583.020 - 200 tests / 2ml
Target species
Human
Specificity
Recognises thrombomodulin (TM), a 105 kDa protein
Clone
B-A35
Application
Flow Cytometry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
TF1 cell line
Quantity
200 Tests / 2ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline with 1 % BSA and 0.09 % sodium azide
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
Use 10 μl to label 106 cells or 100 μl of whole blood
Synonym
Thrombomodulin
Anti-Human CD141 Unconjugated
A typical staining pattern with the B-A35 monoclonal antibody of monocytes

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

CD141 , also known as Thrombomodulin or BDC-A is an transmembrane protein expressed predominantly on the surface of  vascular endothelial cells, but also on other cell types such as macrophages, monocytes, subpopulation of myeloid dendritic cells and keratinocytes. Thrombomodulin is a type 1 membrane glycoprotein of 74kDa consisting on a single chain with 6 Epidermal Growth Factor (EGF)-like domains critical for Thrombin and proteine C binding, and four other domains (an N-terminal C-type lectin domain, a highly glycosylated serine/threonine-rich domain, a transmembrane segment, and  a short C-terminal cytoplasmic tail. It acts as an important component in the anti-coagulation and fibrinolysis system : cofactor for active thrombin, the thrombomodulin-thrombin complex activates protein C to degrade coagulation factors, inhibiting coagulation and fibrinolysis.

When vascular endothelial cells injury occurs, proteolytic cleavage release multiple fragments of thrombomodulin in circulation, so it represents a good marker for endothelial cell damage in pathologies such as cardiovascular disease, acute coronary syndrome, pulmonary thromboembolism or severe hemorrhage. Thrombomodulin has been described as an anticoagulant effect protein, and its therapeutical potential for preventing deep vein thrombosis and its use in the treatment of disseminated intravascular coagulation is under demonstrated in preliminary data.

More recently, other biological effects beyond anticoagulation have been demonstrated such as an anti-inflammatory , cell adhesion and proliferation regulation It is also demonstrated that thrombomodulin may be able to suppress the metastatic capacity of tumor cells, and hence have a protective role in many types of malignancies.

Version 6 - 06.19

For research use only

Documents

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

854.583.020 200 tests / 2ml

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email:

Please contact us to discuss bulk orders