- Catalogue N°
- 854.774.010 - 100 tests / 1ml
- Target species
- Recognises the lipopolysaccharide receptor (LPS R), a 55 kDa protein
- Flow Cytometry
- Myeloma SP2/O x Balb/c spleen cells
- 100 Tests / 1ml
- Mouse IgG2a Kappa light chain
- Lyophilisated in phosphate-buffered saline with 1% BSA and 0.1% sodium azide
- Stable at +2-8°C for six months after reconstitution. For longer storage freeze aliquots.
- Working Dilution
- Reconstitute with 1 ml deionised water. Use 10μl to label 106 cells or 100 μl of whole blood.
Sodium azide is a poisonous and hazardous substance, handle and dispose of properly
CD14 is described as the marker molecule for monocytes and macrophages and as an important component of the innate immune system. It exists in two forms : mCD14 a 55 kDa protein anchored to the membrane by a glycosylphosphoinositol tail (GPI) and two soluble serum proteins (sCD14) with or without the GPT anchor. The mCD14 is critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. CD14 is strongly expressed on cells of the myelomonocytic lineage such as monocytes and osteoclast progenitors and at a lower level on macrophages, Langerhans cells, granulocytes and microglia.
Increased sCD14 levels are associated with inflammatory infectious diseases or allergy.
CD14 is involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes. CD14 is a useful marker for enumeration of monocytes or osteoclast progenitors in peripheral blood and for monocytic and myelomonocytic acute myeloid leukaemia.
Version 4 - 07.19
For research use only
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