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Anti-Human CD138 Unconjugated

Product Specifications

Catalogue N°
854.073.020 - 200 tests / 2ml
Target species
Human
Specificity
Recognises Syndecan-1, the heparan sulfate proteoglycan, a 65-70 kDa protein
Clone
B-A38
Application
Flow Cytometry
Immunohistochemistry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
U266 cell line
Quantity
200 Tests / 2ml (Discovery Size also available please enquire)
Isotype
Mouse IgG1 Kappa light chain
Format
Phosphate-buffered saline with 1% BSA and 0.09% sodium azide
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
Use 10 μl to label 106 cells or 100 μl of whole blood
Synonym
SYNDECAN-1
Anti-Human CD138 Unconjugated
A typical staining pattern with the B-A38 monoclonal antibody of U266 cell line

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

Syndecans are a transmembrane protein family within the heparin sulphate proteoglycan group that interact with many different molecules of the immune system through their heparin sulphate chains. The mammalian syndecan family consists of 4 proteins; syndecan 1 to 4 each encoded by very distinct genes. In adult tissues syndecan 1 (CD138) is predominantly expressed by epithelial cells and plasma cells (both normal and malignant) and currently considered the most reliable surface marker for plasma cells. In addition CD138 is also expressed on pre and immature B cells however this is regulated by IL-6 and LPS stimulation. Syndecan 1 has previously been shown to participate in cell to cell interactions, organ development, vessel formation and tissue regeneration following injury.

CD138 is regularly cleaved from the membrane and as a consequence high levels of soluble CD138 are found in the blood, which can be easily detected using a CD138 specific ELISA.

Via its heparin sulphate chains CD138 binds to and modulates the activity of a wide range of molecules involve in inflammation including chemokines, growth factors, selectins and other adhesion molecules. CD138 can also act as a receptor for collagen, fibronectin, thromobospondin and tenascin therefore involved in cell matrix adhesion. CD138 has been shown to mediate the binding of myeloma cells to type I collagen, and inhibits tumour cell invasion into collagen gels.

As CD138 has been shown to have important effects on tumour cell growth, survival, adhesion and invasion syndecan-1 may be an important regulator in cancer biology.

Version 5 - 06.19

For research use only

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Formats Available

854.073.020 200 tests / 2ml

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