- Catalogue N°
- 953.002.010 - 100 tests / 1ml
- Target species
- Recognises the Fas, APO1 antigen, a 45 kDa protein
- Flow Cytometry
- Myeloma X63/AG.8653 x Balb/c spleen cells
- Recombinant Fas antigen
- 100 Tests / 1ml (Discovery Size also available please enquire)
- Mouse IgG2a Kappa light chain
- Phosphate-buffered saline with 1% BSA and 0.1% sodium azide
- Stable at +2-8°C for six months after reconstitution. DO NOT FREEZE.
- Working Dilution
- Reconstitute with 1 ml deionised water. Use 10μl to label 106 cells or 100 μl of whole blood.
Sodium azide is a poisonous and hazardous substance, handle and dispose of properly
Fas (APO-1, CD95) is a transmembrane protein belonging to the TNF receptor family, capable of mediating apoptotic death of transformed cells as well as activated human T cells. The cell death signal can be induced by anti-Fas antibodies or by the Fas (Fas-L) ligand belonging to the TNF family. The in vivo expression of these two Fas and Fas-L molecules is differently regulated since the expression of Fas is pleiotropic whereas Fas-L is rather expressed in immunity sites (testes, ovaries, brain, eye) and by activated T cells. Tumor cells regardless of their tissue origin may also express Fas-L (pathological).
Genetic and immunological studies have shown that the Fas system is on the one hand involved in the process of peripheral clonal deletion allowing the maintenance of self-tolerance, on the other hand that it regulates the amplitude and duration of immune responses. and finally that it represents one of the mechanisms of lysis of the target cells in the antiviral cytotoxic T response.
When Fas-dependent apoptosis can not occur, due to genetic mutations in the Fas or Fas-L genes, a lymphoproliferative syndrome and autoimmune manifestations are observed in humans and mice. Conversely, an exacerbation of the Fas system may be responsible for tissue destruction. For example, the single mouse injection of anti-Fas antibodies induces massive liver destruction and mouse death within a few hours, leading to the hypothesis of Fas involvement in fulminant hepatitis.
Version 6 - 06.19
For research use only
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