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Anti-Human CD95 FITC Conjugated

Product Specifications

Catalogue N°
953.001.010 - 100 tests / 1ml
Target species
Human
Specificity
Recognises the Fas, APO1 antigen, a 45 kDa protein
Clone
B-G27
Application
Flow Cytometry
Immunohistochemistry
Hybridoma
Myeloma X63/AG.8653 x Balb/c spleen cells
Immunisation
Recombinant Fas antigen
Quantity
100 Tests / 1ml (Discovery Size also available please enquire)
Isotype
Mouse IgG2a Kappa light chain
Format
Phosphate-buffered saline with 5 % BSA and 0.1 % sodium azide
Workshop
VI
Storage
Stable at +2-8°C for 12 months. For longer storage freeze aliquots.
Working Dilution
Use 10 μl to label 106 cells or 100 μl of whole blood
Synonym
APO-1
FAS
Anti-Human CD95 FITC Conjugated
A typical staining pattern with the B-G27 monoclonal antibody of lymphocytes

Sodium azide is a poisonous and hazardous substance, handle and dispose of properly


BACKGROUND

Fas (APO-1, CD95) is a transmembrane protein belonging to the TNF receptor family, capable of mediating apoptotic death of transformed cells as well as activated human T cells. The cell death signal can be induced by anti-Fas antibodies or by the Fas (Fas-L) ligand belonging to the TNF family. The in vivo expression of these two Fas and Fas-L molecules is differently regulated since the expression of Fas is pleiotropic whereas Fas-L is rather expressed in immunity sites (testes, ovaries, brain, eye) and by activated T cells. Tumor cells regardless of their tissue origin may also express Fas-L (pathological).

Genetic and immunological studies have shown that the Fas system is on the one hand involved in the process of peripheral clonal deletion allowing the maintenance of self-tolerance, on the other hand that it regulates the amplitude and duration of immune responses. and finally that it represents one of the mechanisms of lysis of the target cells in the antiviral cytotoxic T response.

When Fas-dependent apoptosis can not occur, due to genetic mutations in the Fas or Fas-L genes, a lymphoproliferative syndrome and autoimmune manifestations are observed in humans and mice. Conversely, an exacerbation of the Fas system may be responsible for tissue destruction. For example, the single mouse injection of anti-Fas antibodies induces massive liver destruction and mouse death within a few hours, leading to the hypothesis of Fas involvement in fulminant hepatitis.

Version 8 - 06.19

For research use only

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Formats Available

953.001.010 100 tests / 1ml

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