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Human CD25 ELISA kit

CE certified product *

Product Specifications

Catalogue N°
950.500.048 - 1 x 48 Discovery (pre-coated)
950.500.096 - 1 x 96 (pre-coated)
950.500.192 - 2 x 96 (pre-coated)
Assay Range
68.75 pg/ml - 2220 pg/ml
Sensitivity
32.5 pg/ml
Target species
Human
Specificity
Recognizes both natural and recombinant human soluble IL-2R
Incubation
3h40
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human soluble receptors
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Synonym
IL-2R
CD25 ELISA Kit
CD25 ELISA Kit

BACKGROUND

IL-2, one of the most important factors in the human immune system, is a potent T-cell growth factor whose major function is the activation of many cells of the immune system including T-cells, B-cells, macrophages and NK cells. These potent actions are mediated by IL-2 binding and signalling through its associated cell surface receptor IL-2R. This receptor is not expressed on normal or unstimulated lymphocytes but is quickly transcribed and expressed on T-cells following activation.

This IL-2R is a heterotrimeric protein consisting of three distinct glycopeptide subunits termed IL-2Ra (CD25) specific to IL-2R, IL-2Rb and IL-2Rg. The a and b chains are involved in binding IL-2 while the signal transduction following IL-2 binding is mediated by the g-chain along with the b chain. The IL-2Ra chain or CD25 is a type 1 transmembrane glycoprotein of 251 amino acids and 55kDa. CD25 can also be found as a soluble form in serum and tissue following enzymatic cleavage from expressing cells and can be identified as a 45KDa protein once shed from the membrane. As the expression and subsequent release of CD25 takes place following cell stimulation the presence of soluble CD25 (sCD25) in circulation is an excellent marker of T-cell activation.

A number of disease states linked to over expression of CD25 have previously been described including, autoimmune diseases, transplant rejection, chronic infection, B-cell neoplasm and various types of leukaemia and other forms of cancer. Because of this definite link between CD25 over expression and disease state many therapies for these conditions have evolved to inhibit this over expression of IL-2Ra.

More recently CD25 has become the major marker for distinguishing the CD4+CD25+ subset of T regulatory cells.

Version 18 - 07.19

For research use only

Documents

* CE marked products are available for IVD use in the EU only. For all other regions these products are for research use only.

For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

950.500.048 1 x 48 Discovery (pre-coated)
950.500.096 1 x 96 (pre-coated)
950.500.192 2 x 96 (pre-coated)

Ordering options

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