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Human TNF-α ELISA Kit

Product Specifications

Catalogue N°
950.090.048 - 1 x 48 Discovery (pre-coated)
950.090.096 - 1 x 96 (pre-coated)
950.090.192 - 2 x 96 (pre-coated)
Assay Range
25 pg/ml - 800 pg/ml
8 pg/ml
Target species
Recognizes both natural and recombinant human TNF-a
Sample Type
Serum, Plasma, Cell culture supernatant
Sample Size
100 µl
Cross Reaction
No cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.
Kit Content
Diaclone ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents and a detailed procedure.
Example of Human TNF-a Standard Curve


Tumor necrosis factor-alpha (TNF-α, also known as TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, cachexin, cachectin or TNFSF2)  is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction.

It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFα is a member of the TNF superfamily, consisting of various transmembrane proteins with a homologous TNF domain

TNF alpha plays a proinflammatory role during a tissue injury or a lesion, sometimes by himself or with mediators like interleukins 1 and 6.

TNF alpha has a cellular intervention where it uses lymphocytes and neutrophils but also permits tissues to remodel and repair.

TNF alpha has a positive action and will protect the body if the immune system is functioning properly. However, if the Tnf-alpha levels are altered it can lead to serious pathologies such as Behçet decease (an inflammation of the blood vessels).

TNF alpha is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.

Version 34 - 05.22

For research use only


For any order, the purchaser acknowledges having read and accepted the terms and conditions described on the Diaclone website.

Formats Available

950.090.048 1 x 48 Discovery (pre-coated)
950.090.096 1 x 96 (pre-coated)
950.090.192 2 x 96 (pre-coated)

Ordering options

Place an order
by phone: +33 (0)3 81 41 38 38
or by email: info [at] diaclone [dot] com ( Subject: Diaclone's order, Body: Human TNF-α ELISA Kit 950.090.048 - 1 x 48 Discovery (pre-coated) 950.090.096 - 1 x 96 (pre-coated) 950.090.192 - 2 x 96 (pre-coated) )

Please contact us to discuss bulk orders